Anti-Cyclin A1抗体[XLA1-3] (ab13337)

概述

  • 产品名称
    Anti-Cyclin A1抗体[XLA1-3]
    参阅全部 Cyclin A1 一抗
  • 描述
    小鼠单克隆抗体[XLA1-3] to Cyclin A1
  • 特异性
    The antibody detects Xenopus cyclin A in blots. Cyclin A1 is the embryonic cyclin which is present in Xenopus eggs. Only detects Xenopus Cyclin A1.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Xenopus laevis
  • 免疫原

    Full length protein.

  • 常规说明
    This product replaces ab10831.

性能

应用

Our Abpromise guarantee covers the use of ab13337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 59 kDa.

靶标

  • 功能
    May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
  • 组织特异性
    Very high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines.
  • 序列相似性
    Belongs to the cyclin family. Cyclin AB subfamily.
  • 发展阶段
    Expression increases in early G1 phase and reaches highest levels during the S and G2/M phases.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • CCN A1 antibody
      • CCNA 1 antibody
      • Ccna1 antibody
      • CCNA1_HUMAN antibody
      • Cyclin-A1 antibody
      • CyclinA1 antibody
      • G2/mitotic specific cyclin A1 antibody
      • MGC132235 antibody
      • MGC159139 antibody
      see all

    实验方案

    文献

    This product has been referenced in:
    • Stiles J  et al. Propranolol treatment of infantile hemangioma endothelial cells: A molecular analysis. Exp Ther Med 4:594-604 (2012). WB . Read more (PubMed: 23170111) »
    • Krasinska L  et al. Cdk1 and Cdk2 activity levels determine the efficiency of replication origin firing in Xenopus. EMBO J 27:758-69 (2008). Xenopus laevis . Read more (PubMed: 18256689) »

    See all 2 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Xenopus laevis Tissue lysate - other (1 embryo per line)
    Loading amount
    1 cells
    Specification
    1 embryo per line
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-12%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Jolanta Kisielewska

    Verified customer

    提交于 Apr 13 2010

    Thank you for your enquiry regarding our cyclin A1 antibodies. Ab13337 is specific against xenopus cyclin A1 as it was raised against the whole protein from xenopus laevis. As there is about 60% homology between human and xenopus cyclin A1 the antibo...

    Read More

    Ab13337 was raised against Xenopus Cyclin A1. If you have any more questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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