概述

  • 产品名称Anti-Cyclin A1抗体
    参阅全部 Cyclin A1 一抗
  • 描述
    兔多克隆抗体to Cyclin A1
  • 特异性Detects endogenous levels of total Cyclin A1 protein.
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • SKOV3 cell extracts.

性能

应用

Our Abpromise guarantee covers the use of ab53699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ELISA 1/40000.
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
  • 组织特异性Very high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines.
  • 序列相似性Belongs to the cyclin family. Cyclin AB subfamily.
  • 发展阶段Expression increases in early G1 phase and reaches highest levels during the S and G2/M phases.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCN A1 antibody
    • CCNA 1 antibody
    • Ccna1 antibody
    • CCNA1_HUMAN antibody
    • Cyclin-A1 antibody
    • CyclinA1 antibody
    • G2/mitotic specific cyclin A1 antibody
    • MGC132235 antibody
    • MGC159139 antibody
    see all

Anti-Cyclin A1 antibody 图像

  • All lanes : Anti-Cyclin A1 antibody (ab53699) at 1/500 dilution

    Lane 1 : SKOV3 cell extract
    Lane 2 : SKOV3 cell extract with immunising peptide


    Predicted band size : 52 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab53699 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53699, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab53699 staining in Human Breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53699, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Cyclin A1 antibody (ab53699)参考文献

This product has been referenced in:
  • Pakula A  et al. Altered expression of cyclin A 1 in muscle of patients with facioscapulohumeral muscle dystrophy (FSHD-1). PLoS One 8:e73573 (2013). ICC/IF . Read more (PubMed: 24019929) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium citrate pH 6.0
Sample Mouse Tissue sections (Testes)
Specification Testes
Blocking step Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Manju Sharma

Verified customer

提交于 Mar 19 2015

Thank you for contacting us.

Your credit note ID is XXXXX.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in on...

Read More

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital ...

Read More

Thank you very much for confirming the details.

We are unsure about the identity of bands. Not many complaints are received for this antibody infact this is the second since December 2011 so protocol troubleshooting might help.

There ...

Read More

Thanks you very much for your email. I am very sorry to hear that you are experiencing problems with this antibody.

I have checked the image and details you have kindly provided and in order to know more about the problem I have following ques...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"