Anti-CYB5R4抗体(ab101342)

概述

  • 产品名称Anti-CYB5R4抗体
    参阅全部 CYB5R4 一抗
  • 描述
    兔多克隆抗体to CYB5R4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 357-508 of Human CYB5R4 (AAH25380).

  • 阳性对照
    • NT2D1, IMR32 and U87-MG cell lines.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab101342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.

靶标

  • 功能NADH-cytochrome b5 reductase involved in endolasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS). Reduces a variety of substrates in vitro, such as cytochrome c, feericyanide and methemoglobin.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
    Contains 1 CS domain.
    Contains 1 cytochrome b5 heme-binding domain.
    Contains 1 FAD-binding FR-type domain.
  • 细胞定位Endoplasmic reticulum. Soluble protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • b5+b5R antibody
    • b5b5r antibody
    • C79736 antibody
    • cb5/cb5R antibody
    • Cb5cb5r antibody
    • Cyb5r4 antibody
    • Cytochrome b type NAD(P)H oxidoreductase antibody
    • Cytochrome b5 reductase 4 antibody
    • dJ676J13.1 antibody
    • Flavohemoprotein b5+b5R antibody
    • Flavohemoprotein b5/b5R antibody
    • N terminal cytochrome b5 and cytochrome b5 oxidoreductase domain containing protein antibody
    • N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein antibody
    • NADPH cytochrome B5 oxidoreductase antibody
    • NB5R4_HUMAN antibody
    • NCB5OR antibody
    • RP4 676J13.1 antibody
    see all

Anti-CYB5R4 antibody 图像

  • Anti-CYB5R4 antibody (ab101342) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size : 59 kDa

Anti-CYB5R4 antibody (ab101342)参考文献

ab101342 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-15%)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Treatment 1 X SDS Loading Buffer
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 17 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step 2% BSA/2% Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (Whole Brain Sagittal)
Specification Whole Brain Sagittal
Permeabilization Yes - 0.1% Triton
Fixative Paraformaldehyde
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Mr. Matt Stroh

Verified customer

提交于 Jan 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"