Anti-CYB5R3抗体[EPR3867] (ab109620)


  • 产品名称Anti-CYB5R3抗体[EPR3867]
    参阅全部 CYB5R3 一抗
  • 描述
    兔单克隆抗体[EPR3867] to CYB5R3
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human CYB5R3.

  • 阳性对照
    • A431, HepG2, and HeLa cell lysates. Human testis tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab109620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
IHC-P 1/250 - 1/500. (Heat to 98°C, allow to cool for 10-20 minutes)
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
    • 组织特异性Isoform 2 is expressed at late stages of erythroid maturation.
    • 疾病相关Defects in CYB5R3 are the cause of methemoglobinemia CYB5R3-related (METHB-CYB5R3) [MIM:250800]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well-tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms.
    • 序列相似性Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
      Contains 1 FAD-binding FR-type domain.
    • 细胞定位Endoplasmic reticulum membrane. Mitochondrion outer membrane and Cytoplasm. Produces the soluble form found in erythrocytes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • B5R antibody
      • Cyb5r3 antibody
      • Cytochrome b5 reductase 3 antibody
      • Cytochrome b5 reductase antibody
      • DIA1 antibody
      • Diaphorase 1 antibody
      • Diaphorase-1 antibody
      • NADH cytochrome b5 reductase 3 antibody
      • NADH-cytochrome b5 reductase 3 membrane-bound form antibody
      • NADH-cytochrome b5 reductase 3 soluble form antibody
      • NB5R3_HUMAN antibody
      • OTTHUMP00000028761 antibody
      • OTTHUMP00000198435 antibody
      • OTTHUMP00000198574 antibody
      • OTTHUMP00000198662 antibody
      • OTTHUMP00000198665 antibody
      see all

    Anti-CYB5R3 antibody [EPR3867] 图像

    • All lanes : Anti-CYB5R3 antibody [EPR3867] (ab109620) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 34 kDa
    • ab109620 at 1/250 dilution staining CYB5R3 in paraffin-embedded Human testis tissue by Immunohistochemistry.

    Anti-CYB5R3 antibody [EPR3867] (ab109620)参考文献

    ab109620 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your inquiry.
    For ab109620, the exact immunogen shares 79% identity with CYB5R2. This antibody is not predicted to recognize CYB5R2, due to low sequence homology but we haven't specifically tested this.
    We do have an CYB5R3 prot...

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