Anti-Cy3 / Cy5抗体[CY96] (ab52060)

概述

  • 产品名称Anti-Cy3 / Cy5抗体[CY96]
  • 描述
    小鼠单克隆抗体[CY96] to Cy3 / Cy5
  • 特异性ab52060 recognizes Cy3, Cy5, AlexaFluor 647 and AlexaFluor 547 conjugated to proteins.
  • 经测试应用适用于: Dot blot, ELISA, IP, ICC, Other, ICC/IFmore details
  • 免疫原

    A mixture of proteins labelled with Cy3 or Cy5.

  • 常规说明


    Monoclonal antibodies to Cy3 and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯化说明Purified Mouse Immunoglobulin
  • Primary antibody说明Monoclonal antibodies to Cy3 and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.
  • 克隆单克隆
  • 克隆编号CY96
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use a concentration of 1 - 2 µg/ml.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Other Use at an assay dependent concentration.

Protein microarrays

ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性Cy3 and Cy5 are reactive watersoluble fluorescent dyes of the cyanine dye family used for labelling proteins, peptides, DNA, RNA, and other biomolecules. They are usually synthesized with reactive groups on either one or both of the nitrogen side chains so that they can be chemically cross-linked to nucleic acids or protein molecules. Thay are small, pH insensitive (between pH 3 and pH 10), soluble in aqueous solution and have tolerance to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. They are used in many different biological assays, including DNA microarrays, protein microarrays, two-dimensional protein analysis, fluorescence resonance energy transfer, and immunocytochemistry. Cy3 is excited maximally at 550 nm and emits maximally at 570 nm, in the green part of the spectrum. Cy5 is excited maximally at 649 nm and emits maximally at 670 nm, in the red part of the spectrum.

Anti-Cy3 / Cy5 antibody [CY96] (ab52060)参考文献

ab52060 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Specification U2OS
Fixative Paraformaldehyde
Permeabilization Yes - 0.2 % Triton X 100 in PBS for 12 min
Blocking step Goat serum in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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提交于 Feb 25 2013

Thank you for contacting us.

Unfortunately we do not have any images of product ab52061 and ab52060 with Alexa 647. Alexa 647 is based on the structure of Cy5 and therefore is structurally very similar. As our data sheet states this antibody...

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Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality...

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Thank you for confirming these details. I am sorry to hear that these antibodies did not work in ICC/IF.
As requested, I have issued a free of charge replacement of ab3851, ab52060, and ab112511. These will be shipped out tonight for delivery tomor...

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Thank you for contacting us. I am sorry we are not expert in this field. However we have few recommendations that will help you to search the right antibodies. I have attached a FRET presentation which I found while searching on Google. Could you p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"