The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Preliminary experiments in Human brain
(cerebellum, hippocampus) lysates gave no specific signal but low background (at antibody concentration up to 1 µg/ml). We would welcome your feedback if you have tested this antibody in more suitable positive controls.
Use a concentration of 3 - 5 µg/ml.
Acts as a negative regulator of the Wnt signaling pathway via its interaction with DVL1.