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Our Abpromise guarantee covers the use of ab8023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 20 µg/ml.|
|WB||1/500. Predicted molecular weight: 44 kDa.Can be blocked with CXCR6 peptide (ab8390).|
ab8023 at 20µg/ml staining CXCR6 in human spleen cells by IHC
ab8023 staining CXCR6 in the Human MCF7 cell line by Flow Cytometry. Samples were incubated with primary antibody (1/50 in PBS) for 20 minutes at 4°C. A FITC-conjugated Pig anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody. The gating strategy was live cells.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"