Anti-CXCR5抗体(ab19017)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to CXCR5
  • 经测试应用
    适用于: Flow Cyt, WBmore details
  • 种属反应性
    与反应: Human, Cynomolgus monkey
  • 免疫原

    Synthetic peptide corresponding to a region near the N terminus of Human CXCR5

性能

应用

Our Abpromise guarantee covers the use of ab19017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 41 kDa).

靶标

  • 功能
    Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
  • 组织特异性
    Expression in mature B-cells and Burkitt lymphoma cells.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BLR 1 antibody
    • BLR1 antibody
    • Burkitt lymphoma receptor 1 antibody
    • Burkitt lymphoma receptor 1 GTP binding protein antibody
    • Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5) antibody
    • C X C chemokine receptor type 5 antibody
    • C-X-C chemokine receptor type 5 antibody
    • CD 185 antibody
    • CD185 antibody
    • CD185 antigen antibody
    • Chemokine (C-X-C motif) receptor 5 antibody
    • Chemokine C X C motif receptor 5 antibody
    • Chemokine CXC motif receptor 5 antibody
    • Chemokine receptor 5 antibody
    • CXC chemokine receptor type 5 antibody
    • CXC R5 antibody
    • CXC-R5 antibody
    • CXCR 5 antibody
    • CXCR-5 antibody
    • Cxcr5 antibody
    • CXCR5_HUMAN antibody
    • MDR 15 antibody
    • MDR-15 antibody
    • MDR15 antibody
    • MGC117347 antibody
    • Monocyte derived receptor 15 antibody
    • Monocyte-derived receptor 15 antibody
    see all

图片

  • Flow cytometry analysis of CXCR5 transfected HEK293 cells, staining CXCR5 with ab19017.

    The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA + 0.01% Azide) for 1 hour at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (200) was used as the secondary antibody.

    Gating Strategy: Live cells

    See Abreview

文献

ab19017 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Dog Cell (blood leucocytes)
Specification
blood leucocytes
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive Lymphocytes
Username

Abcam user community

Verified customer

提交于 Nov 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (human CXCR5 transfected HEK)
Specification
human CXCR5 transfected HEK
Permeabilization
No
Gating Strategy
alive cells
Username

Abcam user community

Verified customer

提交于 Nov 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (Whole blood)
Specification
Whole blood
Preparation
Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

提交于 Apr 19 2012

Application
Flow Cytometry
Sample
Rat Cell (spleen)
Specification
spleen
Preparation
Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

提交于 Apr 19 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spleen from immune mouse)
Specification
Spleen from immune mouse
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 18 2012

Application
Flow Cytometry
Sample
Mouse Cell (splenocytes)
Specification
splenocytes
Preparation
Cell harvesting/tissue preparation method: after spleen mashing
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive B cells
Username

Abcam user community

Verified customer

提交于 Apr 17 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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