概述

  • 产品名称Anti-CXCR4抗体
    参阅全部 CXCR4 一抗
  • 描述
    山羊多克隆抗体to CXCR4
  • 特异性This antibody binds to CXCR 4 receptor on mouse spleen leukocytes as determined by immunocytochemistry.
  • 经测试应用适用于: Flow Cyt, ICC/IF, ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    YSEEVGSGDYDSNKEPCFRDENVHFNR

    , corresponding to N terminal amino acids 14-40 of Mouse CXCR4

  • 阳性对照
    • Mouse spleen cells

应用

Our Abpromise guarantee covers the use of ab1670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 18369102
ICC/IF 1/300.
ELISA 1/50000.
IHC-P 1/300.
WB 1/1000.

靶标

  • 功能Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ions levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhance intracellular calcium ions and reduce cellular cAMP levels. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.
  • 组织特异性Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
  • 疾病相关Defects in CXCR4 are a cause of WHIM syndrome (WHIM) [MIM:193670]; also known as warts, hypogammaglobulinemia, infections and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 结构域The amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity.
  • 翻译后修饰Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation.
    Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S.
    Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization.
    O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity.
  • 细胞定位Cell membrane. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C-X-C chemokine receptor type 4 antibody
    • CD184 antibody
    • CD184 antigen antibody
    • Chemokine (C X C motif) receptor 4 antibody
    • Chemokine CXC Motif Receptor 4 antibody
    • CXC-R4 antibody
    • CXCR-4 antibody
    • CXCR4 antibody
    • CXCR4_HUMAN antibody
    • D2S201E antibody
    • FB22 antibody
    • Fusin antibody
    • HM89 antibody
    • HSY3RR antibody
    • LAP 3 antibody
    • LAP3 antibody
    • LCR1 antibody
    • LESTR antibody
    • Leukocyte derived seven transmembrane domain receptor antibody
    • Leukocyte-derived seven transmembrane domain receptor antibody
    • Lipopolysaccharide associated protein 3 antibody
    • Neuropeptide Y receptor Y3 antibody
    • NPY3R antibody
    • NPYR antibody
    • NPYRL antibody
    • NPYY3 antibody
    • NPYY3R antibody
    • Probable G protein coupled receptor lcr1 homolog antibody
    • SDF 1 receptor antibody
    • SDF-1 receptor antibody
    • SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR antibody
    • Stromal cell derived factor 1 receptor antibody
    • Stromal cell-derived factor 1 receptor antibody
    • WHIM antibody
    • WHIMS antibody
    see all

Anti-CXCR4 antibody 图像

  • ab1670 staining CXCR4 in Mouse Asthma (OVA) lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in PFA and subjected to heat-mediated antigen retrieval in citrate buffer (pH 6.0). The primary antibody was diluted 1/100 in 1% PBS-BSA and incubated with the sample for 16 hours at 4°C. A Biotin-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/500. CXCR4 is revealed in blue (Histogreen) and nuclei are counterstained in red (Neutral Red)

    See Abreview

Anti-CXCR4 antibody (ab1670)参考文献

This product has been referenced in:
  • Zhu Y  et al. Chemokine Signaling Controls Integrity of Radial Glial Scaffold in Developing Spinal Cord and Consequential Proper Position of Boundary Cap Cells. J Neurosci 35:9211-24 (2015). Read more (PubMed: 26085643) »
  • Chen D  et al. Role of medullary progenitor cells in epithelial cell migration and proliferation. Am J Physiol Renal Physiol 307:F64-74 (2014). Read more (PubMed: 24808539) »

See all 7 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Mouse Cell (Cytospined Bone marrow cells)
Permeabilization No
Specification Cytospined Bone marrow cells
Fixative Methanol
Username

Dr. Francois Daubeuf

Verified customer

提交于 Apr 23 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer ph=6
Sample Rat Tissue sections (Kidney)
Specification Kidney
Permeabilization No
Fixative Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

提交于 Mar 05 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer ph=6
Sample Pig Tissue sections (Lung Biopsy)
Specification Lung Biopsy
Permeabilization No
Fixative Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

提交于 Mar 05 2015

Application Flow Cytometry
Fixation None, cells were used fresh
Permeabilization No
Sample Mouse Cell (Cells from bone marrow (C57b6 mice))
Specification Cells from bone marrow (C57b6 mice)
Gating Strategy alive cells (DAPI-), blood cells exluded
Preparation Cell harvesting/tissue preparation method: Bone marrow from Femur was extracted with PBS-EDTA 3mM, 2 hours before flow cytometry analysis
Sample buffer: PBS-EDTA 3mM
Username

Dr. Francois Daubeuf

Verified customer

提交于 Feb 20 2015

I am sorry to read that you are still having trouble detecting CXCR4. Are you confident that the SupT WT are expressing this receptor? I am assuming this is a human cell line.

We have one other recent complaint for this lot (no signal in wes...

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Thank you for your kind comments. It is much appreciated.

If you need any further assistance in the future, please do not hesitate to contact me.

Good luck in your research!

Thank you for confirming the order details.

Your credit note ID is 21712.

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Thank you for your response.

I understand now that previously you have used a different antibody (ab2074) successfully - not the same ab1670.

As our Abpromise indicates, in the event that a product is not functioning in the applicat...

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Thank you for your enquiry regarding ab1670 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

Would you be so kind to confirm the followings: Read More

1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"