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Human CXCR4 transfected NSO mouse myeloma cells.
Our Abpromise guarantee covers the use of ab10403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 0.5 - 5 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml. Fix with 2% formaldehyde and permeabilize with 0.1% saponin.|
|IHC-FoFr||Use at an assay dependent concentration.|
|Flow Cyt||Use 10µg for 106 cells. (see Endres et al reference).
ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|Inhibition Assay||Use a concentration of 2.5 - 20 µg/ml. (this is the ND50 where the antibody will block 50% of the bioactivity due to 0.1 ug/ml recombinant human SDF-1 in an assay measuring chemotaxis using BaF/3 human CXCR-4 transfected cells).|
|Neutralising||Use at an assay dependent concentration. PubMed: 22995914|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"