• 产品名称Anti-CXCR3抗体
    参阅全部 CXCR3 一抗
  • 描述
    兔多克隆抗体to CXCR3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human CXCR3.

    (Peptide available as ab74828.)

  • 阳性对照
    • This antibody gave a positive signal in the following human tissue lysates: Colon, Small Intestine, Lymph Node and Heart IF/ICC: MCF7 cell line




Our Abpromise guarantee covers the use of ab71864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 41 kDa).
ICC/IF Use a concentration of 1 µg/ml.


  • 功能Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
    Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
    Isoform 3 mediates the activity of CXCL11.
  • 组织特异性Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C-X-C chemokine receptor type 3 antibody
    • CD 183 antibody
    • CD182 antibody
    • CD183 antibody
    • Chemokine (C X C motif) receptor 3 antibody
    • Chemokine (C X C) receptor 3 antibody
    • Chemokine CXC Motif Receptor 3 antibody
    • CKR L2 antibody
    • CKR-L2 antibody
    • CKRL2 antibody
    • CMKAR3 antibody
    • CXC-R3 antibody
    • CXCR-3 antibody
    • CXCR3 antibody
    • CXCR3_HUMAN antibody
    • G Protein Coupled Receptor 9 antibody
    • G protein-coupled receptor 9 antibody
    • GPR9 antibody
    • Interferon-inducible protein 10 receptor antibody
    • IP-10 receptor antibody
    • IP10 antibody
    • IP10 R antibody
    • IP10 receptor antibody
    • IP10-R antibody
    • IP10R antibody
    • Mig R antibody
    • Mig receptor antibody
    • Mig-R antibody
    • MIGR antibody
    see all

Anti-CXCR3 antibody 图像

  • ICC/IF image of ab71864 stained formaldehyde cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-CXCR3 antibody (ab71864) at 1 µg/ml

    Lane 1 : Human colon tissue lysate - total protein (ab30051)
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 4 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

    Exposure time : 20 minutes

    Our observed 70kDa band is supported by the following publications: and

Anti-CXCR3 antibody (ab71864)参考文献

ab71864 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Sample Cow Cell (lymphocytes)
Permeabilization No
Gating Strategy lymphocytes
Specification lymphocytes
Preparation Cell harvesting/tissue preparation method: Ficoll gradient
Sample buffer: optimized PBS (BD Cellwash)
Fixation none

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提交于 Sep 18 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cow Tissue sections (spleen, lymphe nodes)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citratbuffer pH6.0 / Tris-EDTA pH 9.0
Permeabilization No
Specification spleen, lymphe nodes
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde

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提交于 Aug 14 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Mouse Cell lysate - whole cell (Endothelcells)
Specification Endothelcells
Treatment Ethanol und Dexametason 100nM 24h
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C

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Verified customer

提交于 May 15 2013