概述

  • 产品名称Anti-CXCR3抗体[49801.111]
    参阅全部 CXCR3 一抗
  • 描述
    小鼠单克隆抗体[49801.111] to CXCR3
  • 特异性This product reacts with CXCR3 transfected cells and not with the parent cell line by flow cytometry. The antibody shows no cross-reactivity with human CXCR1, CXCR2 and CXCR4.
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human CXCR3 transfected NSO mouse myeloma cells.

  • 常规说明


    Chemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The alpha and beta families, with four cysteine residues, are the largest and best characterized. In the alpha family, one amino acid separates the first two cysteine residues (CXC); in the beta family the two cysteine residues (CC) are adjacent to each other. The alpha chemokines that contain the N-terminal Glu-Leu-Arg amino acid sequence (ELR-motif) are chemotactic for neutrophils (such as IL8), while those that do not, act on lymphocytes (such as IP10 and MIG). Examples of chemokines under the beta family category are MCP1-5 and RANTES. The chemokine lymphotactin belongs to the gamma family, with only two cysteines (C), and the recently described fractalkine or neurotactin is a member of the delta family and has the first two cysteine residues separated by three amino-acids (CXXXC). Chemokines bind to specific G protein-coupled cell surface receptors on target cells. Five CXC receptors (CXCR1-5), nine CC receptors (CCR1-9) and one CXXXC receptor (CX3CR1) have been cloned to date. Expression of chemokine receptors can be restricted to some cell types (CXCR1 is expressed in neutrophils) while others (such as CCR2) are expressed in a wide variety of cells. Receptor expression has also been found to be constitutive (including down regulation), inducible or restricted to a cell state of activation. In addition, some chemokine receptors are also expressed in non-hematopoietic cells, such as nerve, endothelial and epithelial cells. This suggests that chemokines have other roles besides leucocyte chemotaxis. CX3CR1, for example, is highly expressed in adult brain. Chemokine receptors are linked to phospholipases through the Gi class of G proteins (inhibition by pertussis toxin). Receptor activation leads to a cascade of cellular events including generation of inositol triphosphate, calcium release and activation of protein kinase C. Chemokine receptors also activate small GTP-binding proteins of the Ras and Rho families, the latter being involved in cell motility events. In addition, chemokines bind to non-signaling molecules such as the Duffy antigen receptor for chemokines (DARC) which may act to remove chemokines from the circulation, and heparan sulfates proteoglycans which may serve to establish an ECM concentration gradient. CXCR3, also known as the IP10/ Mig receptor, shares approximately 40% protein sequence identity with CXCR1 and CXCR2, and 34. 2 -36.9% amino acid sequence identity with the five known CC chemokine receptors 1-5. CXCR3 is highly expressed by IL2 activated T lymphocytes (Th1), but not by resting T lymphocytes, B lymphocytes, monocytes or granulocytes. CXCR3 binds IP10 and Mig (but not PF4) with high affinity and mediates Ca2+ mobilization and chemotaxis. CXCR3 does not bind any of the CXC chemokines containing the ELR motif.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: PBS
    Endotoxin level is < 10 ng per mg antibody as determined by the LAL method.
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明The antibody is purified from ascites fluid using protein G chromatography.
  • Primary antibody说明Chemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The alpha and beta families, with four cysteine residues, are the largest and best characterized. In the alpha family, one amino acid separates the first two cysteine residues (CXC); in the beta family the two cysteine residues (CC) are adjacent to each other. The alpha chemokines that contain the N-terminal Glu-Leu-Arg amino acid sequence (ELR-motif) are chemotactic for neutrophils (such as IL8), while those that do not, act on lymphocytes (such as IP10 and MIG). Examples of chemokines under the beta family category are MCP1-5 and RANTES. The chemokine lymphotactin belongs to the gamma family, with only two cysteines (C), and the recently described fractalkine or neurotactin is a member of the delta family and has the first two cysteine residues separated by three amino-acids (CXXXC). Chemokines bind to specific G protein-coupled cell surface receptors on target cells. Five CXC receptors (CXCR1-5), nine CC receptors (CCR1-9) and one CXXXC receptor (CX3CR1) have been cloned to date. Expression of chemokine receptors can be restricted to some cell types (CXCR1 is expressed in neutrophils) while others (such as CCR2) are expressed in a wide variety of cells. Receptor expression has also been found to be constitutive (including down regulation), inducible or restricted to a cell state of activation. In addition, some chemokine receptors are also expressed in non-hematopoietic cells, such as nerve, endothelial and epithelial cells. This suggests that chemokines have other roles besides leucocyte chemotaxis. CX3CR1, for example, is highly expressed in adult brain. Chemokine receptors are linked to phospholipases through the Gi class of G proteins (inhibition by pertussis toxin). Receptor activation leads to a cascade of cellular events including generation of inositol triphosphate, calcium release and activation of protein kinase C. Chemokine receptors also activate small GTP-binding proteins of the Ras and Rho families, the latter being involved in cell motility events. In addition, chemokines bind to non-signaling molecules such as the Duffy antigen receptor for chemokines (DARC) which may act to remove chemokines from the circulation, and heparan sulfates proteoglycans which may serve to establish an ECM concentration gradient. CXCR3, also known as the IP10/ Mig receptor, shares approximately 40% protein sequence identity with CXCR1 and CXCR2, and 34. 2 -36.9% amino acid sequence identity with the five known CC chemokine receptors 1-5. CXCR3 is highly expressed by IL2 activated T lymphocytes (Th1), but not by resting T lymphocytes, B lymphocytes, monocytes or granulocytes. CXCR3 binds IP10 and Mig (but not PF4) with high affinity and mediates Ca2+ mobilization and chemotaxis. CXCR3 does not bind any of the CXC chemokines containing the ELR motif.
  • 克隆单克隆
  • 克隆编号49801.111
  • 骨髓瘤NS0
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 5µg for 105 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
    Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
    Isoform 3 mediates the activity of CXCL11.
  • 组织特异性Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C-X-C chemokine receptor type 3 antibody
    • CD 183 antibody
    • CD182 antibody
    • CD183 antibody
    • Chemokine (C X C motif) receptor 3 antibody
    • Chemokine (C X C) receptor 3 antibody
    • Chemokine CXC Motif Receptor 3 antibody
    • CKR L2 antibody
    • CKR-L2 antibody
    • CKRL2 antibody
    • CMKAR3 antibody
    • CXC-R3 antibody
    • CXCR-3 antibody
    • CXCR3 antibody
    • CXCR3_HUMAN antibody
    • G Protein Coupled Receptor 9 antibody
    • G protein-coupled receptor 9 antibody
    • GPR9 antibody
    • Interferon-inducible protein 10 receptor antibody
    • IP-10 receptor antibody
    • IP10 antibody
    • IP10 R antibody
    • IP10 receptor antibody
    • IP10-R antibody
    • IP10R antibody
    • Mig R antibody
    • Mig receptor antibody
    • Mig-R antibody
    • MIGR antibody
    see all

Anti-CXCR3 antibody [49801.111] (ab10402)参考文献

This product has been referenced in:
  • de Souza A  et al. Resolution of CD8+ lymphomatoid papulosis after surgical excision of the type AB-thymoma. Am J Dermatopathol 31:475-9 (2009). Read more (PubMed: 19542925) »

See 1 Publication for this product

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