Anti-CXCR3抗体[49801] (ab64714)

概述

  • 产品名称
    Anti-CXCR3抗体[49801]
    参阅全部 CXCR3 一抗
  • 描述
    小鼠单克隆抗体[49801] to CXCR3
  • 特异性
    The clone number has been updated from (2Ar1) to (49801) both clone numbers name the same antibody clone.
  • 经测试应用
    适用于: ICC/IF, IHC-P, IHC-Fr, Neutralising, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    NS0 cells transfected with human CXCR3.

性能

应用

Our Abpromise guarantee covers the use of ab64714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Neutralising Use a concentration of 1 - 6 µg/ml. in the presence of 7ng/ml of rhl-TAC, using the hCXCR3 transfected BaF/3 cells in a chemotaxis assay.
Flow Cyt Use a concentration of 5 - 10 µg/ml. Add 10µl to 1-2.5x105 cells in total reaction volume 200µl.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
    Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
    Isoform 3 mediates the activity of CXCL11.
  • 组织特异性
    Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C-X-C chemokine receptor type 3 antibody
    • CD 183 antibody
    • CD182 antibody
    • CD183 antibody
    • Chemokine (C X C motif) receptor 3 antibody
    • Chemokine (C X C) receptor 3 antibody
    • Chemokine CXC Motif Receptor 3 antibody
    • CKR L2 antibody
    • CKR-L2 antibody
    • CKRL2 antibody
    • CMKAR3 antibody
    • CXC-R3 antibody
    • CXCR-3 antibody
    • CXCR3 antibody
    • CXCR3_HUMAN antibody
    • G Protein Coupled Receptor 9 antibody
    • G protein-coupled receptor 9 antibody
    • GPR9 antibody
    • Interferon-inducible protein 10 receptor antibody
    • IP-10 receptor antibody
    • IP10 antibody
    • IP10 R antibody
    • IP10 receptor antibody
    • IP10-R antibody
    • IP10R antibody
    • Mig R antibody
    • Mig receptor antibody
    • Mig-R antibody
    • MIGR antibody
    see all

图片

  • ab64714 staining CXCR3 in primary cultured HUVEC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/400 in PBS + 2.5% serum) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab64714 staining CXCR3 in pig uterus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to enzymatic antigen retrieval prior to blocking with 10% serum for 20 minutes at 24°C. The primary antibody was diluted 1/200 and incubated with the sample for 30 minutes at 24°C. A biotin-conjugated ABC kit was used to detect the antibody.

    See Abreview

  • ab64714 staining CXCR3 in Human glioblastoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer prior to blocking with a proprietary non-protein reagent for 5 minutes at 25°C. The primary antibody was diluted 1/2000 and incubated with the sample for 30 minutes. A biotin-conjugated goat anti-mouse IgG was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Schludi B  et al. Islet inflammation and ductal proliferation may be linked to increased pancreatitis risk in type 2 diabetes. JCI Insight 2:N/A (2017). IHC-P ; Human . Read more (PubMed: 28679961) »
  • O'Beirne SL  et al. CXCL9 Regulates TGF-ß1-Induced Epithelial to Mesenchymal Transition in Human Alveolar Epithelial Cells. J Immunol 195:2788-96 (2015). Read more (PubMed: 26268659) »

See all 9 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ER1
Permeabilization
No
Specification
Tonsil
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 May 03 2016

Application
Flow Cytometry
Sample
Cow Cell (lymphocytes)
Permeabilization
No
Gating Strategy
lymphocytes
Specification
lymphocytes
Preparation
Cell harvesting/tissue preparation method: Ficoll gradient
Sample buffer: Optimized PBS (BD Cellwash)
Fixation
none
Username

Abcam user community

Verified customer

提交于 Jul 21 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (spleen, lymphe nodes)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0 / Tris-EDTA buffer pH 9.0 / Proteinase K
Permeabilization
No
Specification
spleen, lymphe nodes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 21 2015

Thank you for contacting us.

Both antibodies use PBST or TBST at pH 7.4-7.6 in 5% milk.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your inquiry.

The immunogen for this antibody was the full length human CXCR3 (aa1-368, according to Accession # P49682).

To our knowledge the exact epitope was not further mapped.

I am sorry that I could not...

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Gracias por tu respuesta.
Si os interesa testar el anticuerpo en ratón, lo primero que tenéis que hacer es indicármelo para que yo os envíe el código canjeable por un anticuerpo gratuito en la siguiente compra.
Una vez te mande el código, pod...

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Thank you for contacting us.
Unfortunately we have not tested this antibody in mouse samples so do not have any experimental evidence of the use of it with this species.
The epitope is not mapped, so it is not possible to predict the extent o...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Glioblastoma)
Specification
Glioblastoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
proprietary non-protein reagent as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

提交于 Nov 30 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Uterus)
Specification
Uterus
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Frank White

Verified customer

提交于 Oct 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Primary cultured HUVECs)
Specification
Primary cultured HUVECs
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Bing Wang

Verified customer

提交于 Aug 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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