概述

  • 产品名称
  • 描述
    兔多克隆抗体to CXCR2
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    A synthetic peptide mapping at the middle region of human CXCR2, different from the mouse sequence by six amino acids.

性能

应用

Our Abpromise guarantee covers the use of ab65968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰
    Phosphorylated upon ligand binding; which is required for desensitization.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C-X-C chemokine receptor type 2 antibody
    • CD 182 antibody
    • CD182 antibody
    • CD182 antigen antibody
    • CDw128b antibody
    • Chemokine (CXC) receptor 2 antibody
    • CMKAR2 antibody
    • CXC-R2 antibody
    • CXCR 2 antibody
    • CXCR-2 antibody
    • CXCR2 antibody
    • CXCR2_HUMAN antibody
    • GRO/MGSA receptor antibody
    • High affinity interleukin-8 receptor B antibody
    • IL 8 receptor type 2 antibody
    • IL 8R B antibody
    • IL-8 receptor type 2 antibody
    • IL-8R B antibody
    • IL8 RB antibody
    • IL8 receptor type 2 antibody
    • IL8R B antibody
    • IL8R2 antibody
    • IL8RA antibody
    • Interleukin 8 Receptor B antibody
    • Interleukin 8 receptor, beta antibody
    • Interleukin 8 receptor, type 2 antibody
    see all

Anti-CXCR2 antibody 图像

  • Anti-CXCR2 antibody (ab65968) at 2 µg/ml + Human colon carcinoma lysate

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
  • IHC image of ab65968 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65968, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CXCR2 antibody (ab65968)参考文献

This product has been referenced in:
  • Schludi B  et al. Islet inflammation and ductal proliferation may be linked to increased pancreatitis risk in type 2 diabetes. JCI Insight 2:N/A (2017). IHC-P, WB ; Rat, Human . Read more (PubMed: 28679961) »

See 1 Publication for this product

Product Wall

Thank you for your call today and for letting us know about the trouble with ab14935. As we discussed, I am sending a free of charge vial of ab65968 on the order ***, which should arrive tomorrow. Please keep me updated about the results using this rep...

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (human colon tumor cell-HCT116)
Loading amount
40 µg
Specification
human colon tumor cell-HCT116
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Sep 16 2011

Thank you for submitting an Abreview for ab25968. We greatly appreciate your feedback and strive to publish both negative and positive reviews, rewarding each with Abpoints. Before we publish negative Abreviews our Scientific Support team reviews e...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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