概述

  • 产品名称Anti-CXCR2抗体
    参阅全部 CXCR2 一抗
  • 描述
    兔多克隆抗体to CXCR2
  • 经测试应用适用于: WB, ICC/IFmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CXCR2.

    (Peptide available as ab24280.)

  • 阳性对照
    • In Western Blot, ab21641 gave a positive signal in HL60 whole cell lysate and in human spleen tissue lysate. In IF, this antibody gave a positive signal in HeLa, HepG2 and MCF7 cells.

性能

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应用

Our Abpromise guarantee covers the use of ab21641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ICC/IF Use a concentration of 5 µg/ml.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
    • 序列相似性Belongs to the G-protein coupled receptor 1 family.
    • 翻译后修饰Phosphorylated upon ligand binding; which is required for desensitization.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C-X-C chemokine receptor type 2 antibody
      • CD 182 antibody
      • CD182 antibody
      • CD182 antigen antibody
      • CDw128b antibody
      • Chemokine (CXC) receptor 2 antibody
      • CMKAR2 antibody
      • CXC-R2 antibody
      • CXCR 2 antibody
      • CXCR-2 antibody
      • CXCR2 antibody
      • CXCR2_HUMAN antibody
      • GRO/MGSA receptor antibody
      • High affinity interleukin-8 receptor B antibody
      • IL 8 receptor type 2 antibody
      • IL 8R B antibody
      • IL-8 receptor type 2 antibody
      • IL-8R B antibody
      • IL8 RB antibody
      • IL8 receptor type 2 antibody
      • IL8R B antibody
      • IL8R2 antibody
      • IL8RA antibody
      • Interleukin 8 Receptor B antibody
      • Interleukin 8 receptor, beta antibody
      • Interleukin 8 receptor, type 2 antibody
      see all

    Anti-CXCR2 antibody 图像

    • ICC/IF image of ab21641 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21641, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 and MCF7 cells at 5µg/ml.
    • All lanes : Anti-CXCR2 antibody (ab21641) at 1 µg/ml

      Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
      Lane 2 : Human spleen tissue lysate - total protein (ab29699)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Predicted band size : 41 kDa
      Observed band size : 41 kDa
      Additional bands at : 15 kDa,60 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 12 minutes

    Anti-CXCR2 antibody (ab21641)参考文献

    ab21641 has not yet been referenced specifically in any publications.

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