概述

  • 产品名称
  • 描述
    兔多克隆抗体to CXCL5
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Highly pure (>98%) 93 amino acid recombinant mLIX (mouse Lipopolysaccharide-induced CXC chemokine)

性能

应用

Our Abpromise guarantee covers the use of ab9983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    ELISA:To detect mLIX by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mLIX.

    Western Blot:To detect mLIX by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLIX is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • 靶标

    • 功能
      Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
    • 序列相似性
      Belongs to the intercrine alpha (chemokine CxC) family.
    • 翻译后修饰
      N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AMCFII antibody
      • C-X-C motif chemokine 5 antibody
      • C-X-C motif chemokine ligand 5 antibody
      • chemokine (C-X-C motif) ligand 5 antibody
      • Cxcl5 antibody
      • CXCL5_HUMAN antibody
      • ENA 78 antibody
      • ENA-78 (8-78) antibody
      • ENA-78(1-78) antibody
      • ENA-78(9-78) antibody
      • ENA78 antibody
      • Epithelial derived neutrophil activating protein 78 antibody
      • Epithelial-derived neutrophil-activating protein 78 antibody
      • Lipopolysaccharide-induced CXC chemokine antibody
      • Neutrophil activating peptide ENA 78 antibody
      • Neutrophil-activating peptide ENA-78 antibody
      • neutrophil-activating protein 78 antibody
      • SCYB5 antibody
      • Small inducible cytokine B5 antibody
      • small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78) antibody
      • small inducible cytokine subfamily B, member 5 antibody
      • Small-inducible cytokine B5 antibody
      see all

    图片

    • All lanes : Anti-CXCL5 antibody (ab9983)

      Lane 1 : Marker
      Lane 2 : 250 ng
      Lane 3 : 125 ng
      Lane 4 : 62.5 ng
      Lane 5 : 31.25 ng
      Lane 6 : 15.625 ng
      Lane 7 : 7.8 ng
      Lane 8 : 3.9 ng
      Lane 9 : 1.95 ng
      Lane 10 : 0.975 ng
      Lane 11 : 0.4875 ng
      Lane 12 : 0.24 ng

    实验方案

    文献

    ab9983 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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