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Our Abpromise guarantee covers the use of ab9777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.5 µg/ml.|
|Neutralising||Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of rGRO beta/MIP-2 (100 ng/ml), a concentration of 3.0 - 5.0 µg/ml of this antibody is required.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23776670|
ab9777 staining CXCL2 in Rat lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was blocked with Goat serum, avidin and biotin. Sample was incubated with primary antibody.