概述

  • 产品名称Anti-CXCL14抗体
    参阅全部 CXCL14 一抗
  • 描述
    兔多克隆抗体to CXCL14
  • 经测试应用适用于: IHC-FoFr, IHC-P, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to Human CXCL14 aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab36621)

  • 阳性对照
    • Human Cxcl14/BRAK recombinant protein

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab36622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 22815815
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).

靶标

  • 功能Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactive for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.
  • 组织特异性Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110031L23Rik antibody
    • 1200006I23Rik antibody
    • AI414372 antibody
    • BMAC antibody
    • bolekine antibody
    • BRAK antibody
    • C-X-C motif chemokine 14 antibody
    • Chaemokine, CXC motif, ligand 14 antibody
    • Chemokine (C-X-C motif) ligand 14 antibody
    • Chemokine BRAK antibody
    • CXC chemokine in breast and kidney antibody
    • CXCL14 antibody
    • CXL14_HUMAN antibody
    • JSC antibody
    • Kec antibody
    • Kidney-expressed chemokine CXC antibody
    • KS1 antibody
    • MGC10687 antibody
    • MGC124510 antibody
    • MGC90667 antibody
    • MIP 2 gamma antibody
    • MIP-2G antibody
    • MIP2G antibody
    • MIP2gamma antibody
    • NJAC antibody
    • PRO273 antibody
    • PSEC0212 antibody
    • Scyb14 antibody
    • Small Inducible Cytokine B14 antibody
    • Small inducible cytokine subfamily B (Cys-X-Cys) member 14 (BRAK) antibody
    • Small Inducible Cytokine subfamily B, member 14 antibody
    • Small-inducible cytokine B14 antibody
    • UNQ240 antibody
    see all

Anti-CXCL14 antibody 图像

  • Anti-CXCL14 antibody (ab36622) at 1 µg/ml + Human Cxcl14/BRAK recombinant protein at 0.1 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
  • ab36622, at a 1/50 dilution, staining human CXCL14 in placenta tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

    See Abreview

  • Anti-CXCL14 antibody (ab36622) at 1 µg/ml + Recombinant human CXCL14 protein (ab50043) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 12 minutes
  • Immunohistochemical analysis of inflamed rat dorsal root ganglia, staining CXCL14 with ab36622.

    Dorsal root ganglia were taken 3 days after inflammation, from paraformaldehyde perfused rats and subsequently frozen. Sections were incubated with primary antibody at a dilution of 1/50 overnight at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Anti-CXCL14 antibody (ab36622)参考文献

This product has been referenced in:
  • Strong JA  et al. Microarray analysis of rat sensory ganglia after local inflammation implicates novel cytokines in pain. PLoS One 7:e40779 (2012). IHC-FoFr ; Rat . Read more (PubMed: 22815815) »

See 1 Publication for this product

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Thank you for contacting us.

The protocol that you have referenced was actually provided to us by another customer so I do not have that specific information for you. However the Dako Target Retrieval Solution appears to be a modified citrate...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Placenta)
Specification Placenta
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 May 28 2010

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Cardiac whole homogenates)
Loading amount 20 µg
Specification Cardiac whole homogenates
Gel Running Conditions Reduced Denaturing (10.5)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"