概述

  • 产品名称
  • 描述
    兔多克隆抗体to CX3CR1
  • 特异性
    We have received customer feedback that ab8021 may not be specific for mouse CX3CR1. Because of this we have removed mouse as a guaranteed species. We welcome additional feedback for using this antibody in mouse. Please contact our Scientific Support team for more information.
  • 经测试应用
    适用于: IHC-Fr, ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to N terminal amino acids 2-21 of Human CX3CR1 (Peptide available as ab8125.)

  • 阳性对照
    • Human spleen tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab8021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/100.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Can be blocked with CX3CR1 peptide (ab8125).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
  • 组织特异性
    Expressed in lymphoid and neural tissues.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta chemokine receptor-like 1 antibody
    • C X3 C CKR 1 antibody
    • C-X3-C CKR-1 antibody
    • CCRL1 antibody
    • Chemokine C X3 C motif receptor 1 antibody
    • CMK BRL 1 antibody
    • CMK-BRL-1 antibody
    • CMK-BRL1 antibody
    • CMKBLR1 antibody
    • CMKDR1 antibody
    • CX3C chemokine receptor 1 antibody
    • CX3C CKR1 antibody
    • CX3C1_HUMAN antibody
    • CX3CR1 antibody
    • Fractalkine receptor antibody
    • G-protein coupled receptor 13 antibody
    • GPR13 antibody
    • GPRV28 antibody
    • V28 antibody
    see all

Anti-CX3CR1 antibody 图像

  • Mouse heart tissue stained for CX3CR1 using ab8021 at 2 µg/ml in immunohistochemical analysis.

  • All lanes : Anti-CX3CR1 antibody (ab8021)

    Lane 1 : Human spleen lysate with absence of blocking peptide.
    Lane 2 : Human spleen lysate with presence of blocking peptide.


    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical staining (paraffin embedded) of human heart tissue using anti-CX3CR1 at 2 µg/ml.
  • Immunofluorescence of CX3CR1 in Human Heart cells using ab8021 at 10 ug/ml.

  • Flow cytometry analysis of THP-1 cells using CX3CR1 antibody (ab8021) at 0.1 ug/ml. 

    Green: Isotype control

    Red: CX3CR1 antibody (ab8021) 


  • Developed using the ECL technique

    Performed under reducing conditions.

  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

Anti-CX3CR1 antibody (ab8021)参考文献

This product has been referenced in:
  • Guo X  et al. Hypoxia promotes glioma-associated macrophage infiltration via periostin and subsequent M2 polarization by upregulating TGF-beta and M-CSFR. Oncotarget 7:80521-80542 (2016). IHC, ICC/IF ; Human . Read more (PubMed: 27602954) »
  • Ding XM  et al. Baicalin exerts protective effects against lipopolysaccharide-induced acute lung injury by regulating the crosstalk between the CX3CL1-CX3CR1 axis and NF-?B pathway in CX3CL1-knockout mice. Int J Mol Med 37:703-15 (2016). Read more (PubMed: 26782291) »

See all 30 Publications for this product

Product Wall

Thank you for contacting us. The alignment of the ab8021 immunogen sequence with the protein at the following link showed 90% identity, so we expect the antibody will react with your samples.

http://www.ncbi.nlm.nih.gov/protein/355559765?rep...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Frozen Heart Sections (10um thick sections))
Permeabilization
Yes - 0.3% Trition for 30mins at RT
Specification
Frozen Heart Sections (10um thick sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 17 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Rat spinal cord with lesion)
Specification
Rat spinal cord with lesion
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 03 2015

Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Human Cultured Cells (human microglia cell induced from monocyte)
Specification
human microglia cell induced from monocyte
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 03 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Tissue lysate - whole (Rat spinal cord with lesion on different days)
Specification
Rat spinal cord with lesion on different days
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jan 03 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 8,5
Sample
Human Tissue sections (Heart)
Specification
Heart
Permeabilization
Yes - Wash Buffer from Dako with Tween
Fixative
Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

提交于 May 28 2014

Application
Flow Cytometry
Fixation
Paraformaldehyde
Sample
Mouse Cell (BM)
Specification
BM
Gating Strategy
monocyte
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Apr 25 2014

Thank you for contacting us about the issue you are having with the most recent vial. We have not had any complaints about lot GR82315-1 but if all your reagents and samples are the same as what you used for successful stains, then it is possible you r...

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Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

This antibody was tested for flow cytometry in an outside laboratory, and all of the protocol details are not available. I...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. Your enquiry has been forwarded by #### to the scientific support team and we will be pleased to help you in this...

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1-10 of 31 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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