概述

  • 产品名称Anti-CX3CR1抗体
    参阅全部 CX3CR1 一抗
  • 描述
    兔多克隆抗体to CX3CR1
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide, corresponding to amino acids 175-189 of Human CX3CR 1.

  • 阳性对照
    • THP-1 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab8020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
WB 1/500. Predicted molecular weight: 50 kDa.Can be blocked with CX3CR1 peptide (ab8124).

靶标

  • 功能Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
  • 组织特异性Expressed in lymphoid and neural tissues.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta chemokine receptor-like 1 antibody
    • C X3 C CKR 1 antibody
    • C-X3-C CKR-1 antibody
    • CCRL1 antibody
    • Chemokine C X3 C motif receptor 1 antibody
    • CMK BRL 1 antibody
    • CMK-BRL-1 antibody
    • CMK-BRL1 antibody
    • CMKBLR1 antibody
    • CMKDR1 antibody
    • CX3C chemokine receptor 1 antibody
    • CX3C CKR1 antibody
    • CX3C1_HUMAN antibody
    • CX3CR1 antibody
    • Fractalkine receptor antibody
    • G-protein coupled receptor 13 antibody
    • GPR13 antibody
    • GPRV28 antibody
    • V28 antibody
    see all

Anti-CX3CR1 antibody 图像

  • All lanes : Anti-CX3CR1 antibody (ab8020) at 1/500 dilution

    Lane 1 : THP-1 cell lysate with absence of blocking peptide
    Lane 2 : THP-1 cell lysate with presence of blocking peptide


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • ab8020 at 10µg/ml staining CX3CR1 in human spleen cells by IHC

  • Immunofluorescence of CX3CR1 in THP1 cells using ab8020 at 20 ug/ml.

Anti-CX3CR1 antibody (ab8020)参考文献

This product has been referenced in:
  • Szukiewicz D  et al. CX3CL1 (fractalkine) and TNFa production by perfused human placental lobules under normoxic and hypoxic conditions in vitro: the importance of CX3CR1 signaling. Inflamm Res 63:179-89 (2014). IHC-P ; Human . Read more (PubMed: 24270813) »
  • Rooper L  et al. Fractalkine receptor is expressed in mature ovarian teratomas and required for epidermal lineage differentiation. J Ovarian Res 6:57 (2013). Read more (PubMed: 23958497) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain tissue)
Permeabilization Yes - 0.5% triton
Specification brain tissue
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Formaldehyde
Username

Dr. Ali Ertürk

Verified customer

提交于 Oct 24 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cell (Brain slice - CA1 hippocampal section)
Permeabilization Yes - with blocking
Specification Brain slice - CA1 hippocampal section
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 13 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Adult mouse brain. LANE NUMBER 2 IN THE FIGURE.)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 80 µg
Specification Adult mouse brain. LANE NUMBER 2 IN THE FIGURE.
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 02 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tooth)
Antigen retrieval step None
Permeabilization No
Specification tooth
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 30 2015

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (gel 10%)
Sample Mouse Cell lysate - whole cell (Epithelial ovarian cancer cell line)
Specification Epithelial ovarian cancer cell line
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Hilal Gurler

Verified customer

提交于 Jun 18 2013

Thank you for your enquiry. As far as we are aware, cross reactivity with mouse has not yet been tested for use with ab7201. Also, ab8020 has not yet been tested for application in IHC-frozen sections (the antibody has only so far been tested in Wester...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"