概述

  • 产品名称Anti-CX3CL1抗体
    参阅全部 CX3CL1 一抗
  • 描述
    兔多克隆抗体to CX3CL1
  • 经测试应用适用于: ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment: E. coli expressed chemotactic domain of human CX3CL1

性能

应用

Our Abpromise guarantee covers the use of ab25088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22387113
IHC-Fr 1/100 - 1/500.
IHC-P Use a concentration of 4 µg/ml.
WB 1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
IP 1/300 - 1/800.

靶标

  • 功能The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.
  • 组织特异性Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas.
  • 序列相似性Belongs to the intercrine delta family.
  • 翻译后修饰A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A 152E5.2 antibody
    • AB030188 antibody
    • ABCD 3 antibody
    • ABCD3 antibody
    • AI848747 antibody
    • C-X3-C motif chemokine 1 antibody
    • C3Xkine antibody
    • Chemokine (C-X3-C motif) ligand 1 antibody
    • Chemokine C X3 C motif ligand 1 antibody
    • Chemokine CX3C Motif Ligand 1 antibody
    • CX3C membrane anchored chemokine antibody
    • CX3C membrane-anchored chemokine antibody
    • Cx3cl1 antibody
    • CXC 3 antibody
    • CXC3 antibody
    • CXC3C antibody
    • D8Bwg0439e antibody
    • FKN antibody
    • Fractalkine antibody
    • Neurotactin antibody
    • NTN antibody
    • NTT antibody
    • Processed fractalkine antibody
    • SCYD 1 antibody
    • SCYD1 antibody
    • Small inducible cytokine D1 antibody
    • Small inducible cytokine subfamily D (Cys X3 Cys) member 1 antibody
    • small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin) antibody
    • Small inducible cytokine subfamily D member 1 antibody
    • Small-inducible cytokine D1 antibody
    • X3CL1_HUMAN antibody
    see all

Anti-CX3CL1 antibody 图像

  • Anti-CX3CL1 antibody (ab25088) at 1/1000 dilution + mouse recombinant protein at 0.025 µg

    Secondary
    HRP conjugated goat anti-rabbit at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab25088 (4µg/ml) staining CX3CL1 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasmic region of the cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab25088 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25088, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CX3CL1 antibody (ab25088)参考文献

This product has been referenced in:
  • Ruchaya PJ  et al. The cardiovascular actions of fractalkine/CX3CL1 in the hypothalamic paraventricular nucleus are attenuated in rats with heart failure. Exp Physiol 99:111-22 (2014). Rat . Read more (PubMed: 24036597) »
  • Carrega P  et al. CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph. J Immunol 192:3805-15 (2014). Human . Read more (PubMed: 24646734) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Primary astrocytes)
Loading amount 2 µg
Specification Primary astrocytes
Gel Running Conditions Reduced Denaturing (gel 10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 16 2013

Thank you for contacting us. We consider IHC-P to be staining of tissue in paraffin embedded tissue sections. We use the term immunofluorescence or (or immunocytochemistry) to mean staining of cells.

I hope this information is helpful to you...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (endothelial cell)
Loading amount 80000 cells
Specification endothelial cell
Gel Running Conditions Reduced Denaturing (gel 6%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (endothelial cell)
Specification endothelial cell
Fixative Paraformaldehyde
Permeabilization Yes - Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Recombinant protein (recombinant protein)
Loading amount 0.025 µg
Specification recombinant protein
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 16 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"