Anti-Cullin 7抗体(ab34841)

概述

  • 产品名称
    Anti-Cullin 7抗体
    参阅全部 Cullin 7 一抗
  • 描述
    兔多克隆抗体to Cullin 7
  • 特异性
    Ab34841 is recognizes Cullin 7, but cross reactivity with other human cullins may occur.
  • 经测试应用
    适用于: IP, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    RSRGPVYASCTATQSFSTFR

    conjugated to KLH, corresponding to C terminal amino acids 1679 - 1698 of Human Cullin 7.

  • 阳性对照
    • IP: Protein from cell lysates (using HeLa, NIH-3T3, and others). Most cell lines expressing Cullin 7 can be used as a positive control.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: Whole serum
  • Concentration information loading...
  • 纯化说明
    Ab34841 is a monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab34841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
ELISA
WB
  • 应用说明
    ELISA: 1/2000 - 1/10000.
    IP: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Component of a probable SCF-like E3 ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably plays a role in the degradation of proteins involved in endothelial proliferation and/or differentiation (By similarity). Seems not to promote polyubiquitination and proteasomal degradation of TP53. In vitro, complexes of CUL7 with either CUL9 or FBXW8 or TP53 contain E3 ubiquitin-protein ligase activity.
    • 组织特异性
      Highly expressed in fetal kidney and adult skeletal muscle. Also abundant in fetal brain, as well as in adult pancreas, kidney, placenta and heart. Detected in trophoblasts, lymphoblasts, osteoblasts, chondrocytes and skin fibroblasts.
    • 通路
      Protein modification; protein ubiquitination.
    • 疾病相关
      Defects in CUL7 are the cause of 3M syndrome type 1 (3M1) [MIM:273750]. An autosomal recessive disorder characterized by severe pre- and postnatal growth retardation, facial dysmorphism, large head circumference, and normal intelligence and endocrine function. Skeletal changes include long slender tubular bones and tall vertebral bodies.
    • 序列相似性
      Belongs to the cullin family.
      Contains 1 DOC domain.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CUL-7 antibody
      • CUL7 antibody
      • CUL7_HUMAN antibody
      • Cullin-7 antibody
      • dJ20C7.5 antibody
      • KIAA0076 antibody
      see all

    Anti-Cullin 7 antibody (ab34841)参考文献

    ab34841 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Jurkat cells)
    Loading amount
    100000 cells
    Specification
    Jurkat cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 May 12 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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