概述

  • 产品名称Anti-Cullin 4a抗体
    参阅全部 Cullin 4a 一抗
  • 描述
    兔多克隆抗体to Cullin 4a
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Chicken, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Sea Urchin, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal residues 700 - 759) of Human Cullin 4a (NP_001008895.1)

  • 阳性对照
    • Human breast tumor; Mouse squamous cell carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab84382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: 1/100 - 1/500. Epitope exposure with citrate buffer will enhance staining.
    Note: expected to work on frozen secions.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Belongs to the cullin family.
    • 翻译后修饰Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity). Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2810470J21Rik antibody
      • AW495282 antibody
      • CUL 4A antibody
      • CUL-4A antibody
      • Cul4a antibody
      • Cul4a protein antibody
      • CUL4A_HUMAN antibody
      • Cullin-4A antibody
      • MGC36573 antibody
      • MGC64071 antibody
      see all

    Anti-Cullin 4a antibody 图像

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast tumor tissue using 1/500 ab84382 followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse squamous cell carcinoma tissue using 1/100 ab84382, followed by DAB staining.

    Anti-Cullin 4a antibody (ab84382)参考文献

    ab84382 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab84382.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"