概述

  • 产品名称Anti-Cullin 3抗体
    参阅全部 Cullin 3 一抗
  • 描述
    兔多克隆抗体to Cullin 3
  • 经测试应用适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide, corresponding to amino acids 718-768 of Human Cullin 3 (NP_003581.1)

  • 阳性对照
    • HeLa whole cell lysate NIH3T3 whole cell lysate 293T whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab72187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition.
    • 组织特异性Widely expressed.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Belongs to the cullin family.
    • 翻译后修饰Neddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
    • 细胞定位Nucleus. Golgi apparatus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CUL 3 antibody
      • Cul-3 antibody
      • cul3 antibody
      • CUL3_HUMAN antibody
      • Cullin-3 antibody
      • Cullin3 antibody
      • KIAA0617 antibody
      • PHA2E antibody
      see all

    Anti-Cullin 3 antibody 图像

    • All lanes : Anti-Cullin 3 antibody (ab72187) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 89 kDa
      Observed band size : 89 kDa


      Exposure time : 3 minutes

    Anti-Cullin 3 antibody (ab72187)参考文献

    This product has been referenced in:
    • Gastaldello S  et al. Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells. PLoS Pathog 9:e1003664 (2013). Human . Read more (PubMed: 24130483) »

    See 1 Publication for this product

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