Anti-Cullin 3/CUL-3抗体(ab118960)
Key features and details
- Rabbit polyclonal to Cullin 3/CUL-3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cullin 3/CUL-3抗体
参阅全部 Cullin 3/CUL-3 一抗 -
描述
兔多克隆抗体to Cullin 3/CUL-3 -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Cullin 3/CUL-3 aa 1-100 conjugated to keyhole limpet haemocyanin.
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阳性对照
- Human breast tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 6
Preservative: 0.01% Sodium azide -
Concentration information loading...
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纯化说明
Delipidation and Defibrination -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118960于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. -
组织特异性
Widely expressed. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the cullin family. -
翻译后修饰
Neddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. -
细胞定位
Nucleus. Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 8452 Human
- Entrez Gene: 26554 Mouse
- Entrez Gene: 301555 Rat
- Omim: 603136 Human
- SwissProt: Q13618 Human
- SwissProt: Q9JLV5 Mouse
- SwissProt: B5DF89 Rat
- Unigene: 372286 Human
see all -
别名
- CUL 3 antibody
- Cul-3 antibody
- cul3 antibody
see all
图片
数据表及文件
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Datasheet download
文献 (0)
ab118960 尚未被引用在任何文献中。