概述

  • 产品名称Anti-Cullin 2抗体
    参阅全部 Cullin 2 一抗
  • 描述
    兔多克隆抗体to Cullin 2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide from the C-terminus of human Cullin 2

  • 阳性对照
    • Raji cells. Tonsil.

性能

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应用

Our Abpromise guarantee covers the use of ab74280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: 1/200 for 10 min at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 mins, followed by cooling at RT for 20 mins.
    WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at RT. Predicted molecular weight: 87 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Belongs to the cullin family.
    • 翻译后修饰CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cul2 antibody
      • CUL 2 antibody
      • CUL-2 antibody
      • CUL2 antibody
      • CUL2_HUMAN antibody
      • cullin 2 antibody
      • cullin homolog 2 antibody
      • Cullin-2 antibody
      • MGC131970 antibody
      see all

    Anti-Cullin 2 antibody 图像

    • Staining of formalin-fixed, paraffin-embedded human tonsil tissue using 1/200 ab74280.

    Anti-Cullin 2 antibody (ab74280)参考文献

    ab74280 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"