概述

  • 产品名称Anti-Cullin 2抗体
    参阅全部 Cullin 2 一抗
  • 描述
    小鼠多克隆抗体to Cullin 2
  • 经测试应用适用于: IP, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Orangutan
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to internal sequence amino acids 161-260 of Human Cullin 2. Target sequence used ot make the antibody: RMLLREIKND RGGEDPNQKV IHGVINSFVH VEQYKKKFPL KFYQEIFESP FLTETGEYYK QEASNLLQES NCSQYMEKVL GRLKDEEIRC RKYLHPSSYT.

性能

应用

Our Abpromise guarantee covers the use of ab43562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
WB
  • 应用说明IP: Use at an assay dependent dilution (PMID 19622774).
    WB: 1/1000. Predicted molecular weight: 87 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Belongs to the cullin family.
    • 翻译后修饰CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cul2 antibody
      • CUL 2 antibody
      • CUL-2 antibody
      • CUL2 antibody
      • CUL2_HUMAN antibody
      • cullin 2 antibody
      • cullin homolog 2 antibody
      • Cullin-2 antibody
      • MGC131970 antibody
      see all

    Anti-Cullin 2 antibody 图像

    • All lanes : Anti-Cullin 2 antibody (ab43562) at 1/1000 dilution

      Lane 1 : Total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
      Lane 2 : Total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein).

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM, HRP conjugated, at 1/5000 dilution

      Predicted band size : 87 kDa

    Anti-Cullin 2 antibody (ab43562)参考文献

    This product has been referenced in:
    • Ambrosini G  et al. Differentiation-related gene-1 decreases Bim stability by proteasome-mediated degradation. Cancer Res 69:6115-21 (2009). IP ; Human . Read more (PubMed: 19622774) »

    See 1 Publication for this product

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