概述

  • 产品名称Anti-CUG-BP1抗体[3B1]
    参阅全部 CUG-BP1 一抗
  • 描述
    小鼠单克隆抗体[3B1] to CUG-BP1
  • 经测试应用适用于: IHC-FoFr, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Cow, Human, Pig
  • 免疫原

    CUG-BP1 human nuclear RNA binding protein.

  • 阳性对照
    • WB: HeLa WCL, SHSY-5Y WCL, Human kidney tissue lysate IF/ICC: MCF7 IHC: Human Breast cancer tissue

性能

应用

Our Abpromise guarantee covers the use of ab9549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr
ICC/IF
WB
  • 应用说明IHC-FoFr: Use at a concentration of 2 µg/ml.
    IF: 1/500.
    WB: 1/500. Detects a band of approximately 50 kDa in HeLa lysates(predicted molecular weight: 54 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver.
    • 组织特异性Ubiquitous.
    • 序列相似性Belongs to the CELF/BRUNOL family.
      Contains 3 RRM (RNA recognition motif) domains.
    • 翻译后修饰Phosphorylated. Its phosphorylation status increases in senescent cells.
    • 细胞定位Nucleus. Cytoplasm. RNA-binding activity is detected in both nuclear and cytoplasmic compartments.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 50 kDa Nuclear polyadenylated RNA binding protein antibody
      • 50 kDa nuclear polyadenylated RNA-binding protein antibody
      • Bruno like 2 antibody
      • bruno like protein 2 antibody
      • Bruno-like protein 2 antibody
      • BRUNOL 2 antibody
      • BRUNOL2 antibody
      • CELF 1 antibody
      • CELF-1 antibody
      • celf1 antibody
      • CELF1 CUGBP, Elav like family member 1 antibody
      • CELF1_HUMAN antibody
      • CUG BP and ETR 3 like factor 1 antibody
      • CUG BP antibody
      • CUG BP1 antibody
      • CUG RNA binding protein antibody
      • CUG triplet repeat RNA binding protein 1 antibody
      • CUG triplet repeat RNA-binding protein 1 antibody
      • CUG-BP antibody
      • CUG-BP- and ETR-3-like factor 1 antibody
      • CUG-BP1 antibody
      • CUGBP 1 antibody
      • CUGBP and ETR3 like factor 1 antibody
      • CUGBP antibody
      • CUGBP Elav like family member 1 antibody
      • CUGBP Elav-like family member 1 antibody
      • CUGBP1 antibody
      • Cytidine uridine guanosine binding protein 1 antibody
      • Deadenylation factor CUG BP antibody
      • Deadenylation factor CUG-BP antibody
      • Deadenylation factor CUGBP antibody
      • EDEN BP antibody
      • EDEN BP homolog antibody
      • EDEN-BP antibody
      • EDEN-BP homolog antibody
      • embryo deadenylation element binding protein antibody
      • embryo deadenylation element binding protein homolog antibody
      • Embryo deadenylation element-binding protein homolog antibody
      • hNab 50 antibody
      • hNab50 antibody
      • NAB 50 antibody
      • NAB50 antibody
      • NAPOR antibody
      • Nuclear polyadenylated RNA binding protein 50 kD antibody
      • Nuclear polyadenylated RNA binding protein antibody
      • RNA binding protein BRUNOL 2 antibody
      • RNA binding protein BRUNOL2 antibody
      • RNA-binding protein BRUNOL-2 antibody
      see all

    Anti-CUG-BP1 antibody [3B1] 图像

    • All lanes : Anti-CUG-BP1 antibody [3B1] (ab9549) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
      Lane 3 : Human kidney tissue lysate - total protein (ab30203)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 54 kDa
      Observed band size : 54 kDa


      Exposure time : 20 minutes
    • ab9549 stained MCF7 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9549 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • IHC image of CUG-BP1 staining in human normal kidney formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9549, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    Anti-CUG-BP1 antibody [3B1] (ab9549)参考文献

    This product has been referenced in:
    • Ketley A  et al. High-content screening identifies small molecules that remove nuclear foci, affect MBNL distribution and CELF1 protein levels via a PKC-independent pathway in myotonic dystrophy cell lines. Hum Mol Genet N/A:N/A (2013). WB ; Human . Read more (PubMed: 24179176) »
    • Tang ZZ  et al. Muscle weakness in myotonic dystrophy associated with misregulated splicing and altered gating of Ca(V)1.1 calcium channel. Hum Mol Genet 21:1312-24 (2012). WB ; Mouse . Read more (PubMed: 22140091) »

    See all 8 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (fetal lung)
    Loading amount 20 µg
    Specification fetal lung
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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    提交于 Feb 20 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"