Anti-CUEDC2抗体(ab79036)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to CUEDC2
  • 特异性
    ab79036 will not cross-react with CUEDC1
  • 经测试应用
    适用于: ICC/IF, IP, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 19 amino acid synthetic peptide from near the carboxy terminus of human CUEDC2 (NP_076945).

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab79036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 32 kDa.
ELISA Use at an assay dependent dilution.

靶标

图片

  • Lane 1 : Anti-CUEDC2 antibody (ab79036) at 1 µg/ml
    Lane 2 : Anti-CUEDC2 antibody (ab79036) at 2 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 32 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

文献

ab79036 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Raw 264.7)
Loading amount
25 µg
Specification
Raw 264.7
Treatment
shRNA control or against CUEDC2
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jul 31 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (Raw 264.7)
Total protein in input
200 µg
Specification
Raw 264.7
Treatment
2hrs LPS
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

提交于 Jul 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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