Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
A region within synthetic peptide: RDFLLALERD RLKYESQKSK SSSVAVGNDF GFSSPVPGTG DANPAVSEDA, corresponding to internal sequence amino acids 253-302 of Human CUEDC1
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 2.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
The function of this protein remains unknown. It contains 1 CUE domain.
- CUE domain containing protein 1 antibody
- CUEDC1 antibody
Western blot - Anti-CUEDC1 antibody (ab58696)
Anti-CUEDC1 antibody (ab58696) at 2.5 µg/ml + HepG2 cell lysate at 10 µg with skimmed milk in PBS buffer at 5 %Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
42 kDaObserved band size:
48 kDa (why is the actual band size different from the predicted?
Gel concentration 12%
has not yet been referenced specifically in any publications.
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