Anti-CTRP5抗体(ab36893)

概述

  • 产品名称Anti-CTRP5抗体
    参阅全部 CTRP5 一抗
  • 描述
    兔多克隆抗体to CTRP5
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 15 amino acid peptide from near the amino terminus of human CTRP5.

  • 阳性对照
    • Human brain cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab36893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 25 kDa).Can be blocked with Human CTRP5 peptide (ab39744).
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 相关性Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. CTRP5 has been suggested to be involved in age-related macular degeneration.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • C1q and tumor necrosis factor related protein 5 antibody
    • C1QTNF5 antibody
    • Complement C1q tumor necrosis factor related protein 5 precursor antibody
    • LORD antibody

Anti-CTRP5 antibody 图像

  • ab36893 staining CTRP5 in human pancreas carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in 10mM sodium citrate + 0.05% Tween. Samples were incubated with primary antibody (1/750 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Lane 1 : Anti-CTRP5 antibody (ab36893) at 1 µg/ml
    Lane 2 : Anti-CTRP5 antibody (ab36893) at 2 µg/ml
    Lane 3 : Anti-CTRP5 antibody (ab36893) at 4 µg/ml

    Lane 1 : Human brain cell lysate
    Lane 2 : Human brain cell lysate
    Lane 3 : Human brain cell lysate

    Lysates/proteins at 15 µg/ml per lane.

    Secondary
    Anti-rabbit IgG HRP at 1/10000 dilution

    Predicted band size : 25 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Lanes read from left to right, 1-3. These proteins are often highly modified post-translationally and may migrate in SDS-PAGE at positions other than their predicted size.
  • ab36993 at 10µg/ml staining CTRP5 in human brain tissue by IHC

Anti-CTRP5 antibody (ab36893)参考文献

ab36893 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Human Tissue sections (human colon muscle)
Specification human colon muscle
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Mrs. Maria Cecilia Ybanez

Verified customer

提交于 Apr 07 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate, 0.05% Tween
Sample Human Tissue sections (human pancreas carcinoma)
Specification human pancreas carcinoma
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

提交于 Mar 17 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Mouse Tissue sections (mouse pancreas)
Specification mouse pancreas
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

提交于 Nov 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"