Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human CTRP1.
(Peptide available as ab94377.)
- This antibody gave a positive signal in the following lysates:
HEK293 whole cell; A498 whole cell; MDA-MB-361 whole cell; JEG-3 whole cell; Human Placenta Tissue
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Contains 1 C1q domain.
Contains 1 collagen-like domain.
Information by UniProt
- C1q and tumor necrosis factor related protein 1 antibody
- C1QT1_HUMAN antibody
- C1QTNF1 antibody
Western blot - CTRP1 antibody (ab83177)
All lanes :
Anti-CTRP1 antibody (ab83177) at 1 µg/mlLane 1 :
HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 2 :
A498 (Human Kidney Carcinoma) Whole Cell Lysate Lane 3 :
MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate Lane 4 :
JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate Lane 5 :
Human placenta tissue lysate - total protein (ab29745
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
32 kDaObserved band size:
37 kDa (why is the actual band size different from the predicted?
)Additional bands at:
21 kDa. We are unsure as to the identity of these extra bands.
CTRP1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"