Anti-CTNNBL1抗体(ab95170)
Key features and details
- Rabbit polyclonal to CTNNBL1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CTNNBL1抗体
参阅全部 CTNNBL1 一抗 -
描述
兔多克隆抗体to CTNNBL1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MDVGELLSYQ PNRGTKRPRD DEEEEQKMRR KQTGTRERGR YREEEMTVVE) of Human CTNNBL1 (NP_110517.2).
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阳性对照
- HeLa, 293T and NIH-3T3 whole cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95170于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 65 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Induces apoptosis in CHO cells. -
组织特异性
Widely expressed with highest levels in skeletal muscle, placenta, heart, spleen, testis and thyroid. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 282421 Cow
- Entrez Gene: 56259 Human
- Entrez Gene: 66642 Mouse
- Entrez Gene: 296320 Rat
- Omim: 611537 Human
- SwissProt: O62703 Cow
- SwissProt: Q8WYA6 Human
- SwissProt: Q9CWL8 Mouse
see all -
别名
- 5730471K09Rik antibody
- Beta catenin like 1 antibody
- Beta catenin like protein 1 antibody
see all
图片
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All lanes : Anti-CTNNBL1 antibody (ab95170) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 65 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling CTNNBL1 with ab95170 at 1/200 (1µg/ml). Detection: DAB.
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Detection of CTNNBL1 in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab95170 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with exposure time of 3 minutes.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab95170 被引用在 1 文献中.
- Dong B et al. Detection of RACK1 and CTNNBL1-induced activation of mouse splenocytes using an immunoprecipitation-based technique. Mol Med Rep 16:7056-7063 (2017). PubMed: 28901482