概述

  • 产品名称Anti-CTNNBIP1抗体
    参阅全部 CTNNBIP1 一抗
  • 描述
    小鼠单克隆抗体to CTNNBIP1
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: MNREGAPGKS PEEMYIQQKV RVLLMLRKMG SNLTASEEEF LRTYAGVVNS QLSQLPPHSI DQGAEDVVMA FSRSETEDRR Q, corresponding to amino acids 1-82 of Human CTNNBIP1

性能

应用

Our Abpromise guarantee covers the use of ab57544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Prevents the interaction between CTNNB1 and TCF family members, and acts as negative regulator of the Wnt signaling pathway.
  • 序列相似性Belongs to the CTNNBIP1 family.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta catenin interacting protein 1 antibody
    • Beta catenin interacting protein ICAT antibody
    • Beta-catenin-interacting protein 1 antibody
    • Catenin beta interacting protein 1 antibody
    • CNBP1_HUMAN antibody
    • CTNNBIP 1 antibody
    • CTNNBIP1 antibody
    • ICAT antibody
    • Inhibitor of beta catenin and Tcf 4 antibody
    • Inhibitor of beta-catenin and Tcf-4 antibody
    • MGC15093 antibody
    see all

Anti-CTNNBIP1 antibody 图像

  • Anti-CTNNBIP1 antibody (ab57544) + immunogen

    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab57544 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57544, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-CTNNBIP1 antibody (ab57544)参考文献

ab57544 has not yet been referenced specifically in any publications.

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