The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/5000 - 1/15000. Detects a band of approximately 120 kDa (predicted molecular weight: 99 kDa).
Use at 1-5 µg/mg of lysate.
Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
All lanes: 1mg of nuclear extract from Jurkat cell treated with ab70452 at 0.5µg/ml.
Lane 1: ab70452 used at 1 µg/mg lysate for IP.
Lane 2: ab70452 used at 10 µg/mg lysate for IP.
Lane 3: an alternate Ctip2 antibody (ab70453) used at 1 µg/mg lysate for IP.
Lane 4: an alternate Ctip2 antibody (ab70453) used at 10 µg/mg lysate for IP.
Normal rabbit IgG was used as a control for immunoprecipitation reactions.
Chemiluminescence with exposure time of 30 seconds.