Anti-Ctip2抗体[25B6] - ChIP Grade (ab18465)

概述

  • 产品名称Anti-Ctip2抗体[25B6] - ChIP Grade
    参阅全部 Ctip2 一抗
  • 描述
    大鼠单克隆抗体[25B6] to Ctip2 - ChIP Grade
  • 特异性Detects 2 bands representing Ctip2 at about 120kD. Ctip2 is highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • 经测试应用适用于: ICC/IF, IHC-FoFr, WB, IP, IHC-P, IHC-Fr, ChIP, IHC-FrFl, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Human, Zebrafish, Cynomolgus Monkey
  • 免疫原

    Fusion protein corresponding to Human Ctip2.

  • 表位Between amino acids 1-150 of CTIP2.
  • 阳性对照
    • WB: nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody IHC: adult mouse hippocampus, Adult mouse spinal cord ICC: Neonatal mouse hippocampal cultured neurons
  • 常规说明

    Hybridoma produced by fusion of a rat lymphocyte and mouse myeloma.

性能

应用

Our Abpromise guarantee covers the use of ab18465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500.
IHC-FoFr Use at an assay dependent concentration. PubMed: 18523013
WB Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 95 kDa).
IP Use at an assay dependent concentration.
IHC-P 1/500.
IHC-Fr 1/500.
ChIP Use at an assay dependent concentration.
IHC-FrFl Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
  • 组织特异性Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • 序列相似性Contains 6 C2H2-type zinc fingers.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATL1 alpha antibody
    • ATL1 antibody
    • ATL1 beta antibody
    • ATL1 gamma antibody
    • ATL1-delta antibody
    • B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein antibody
    • B cell lymphoma/leukemia 11B antibody
    • B-cell CLL/lymphoma 11B (zinc finger protein) antibody
    • B-cell CLL/lymphoma 11B antibody
    • B-cell lymphoma/leukemia 11B antibody
    • BC11B_HUMAN antibody
    • BCL-11B antibody
    • Bcl11b antibody
    • BCL11B/TRDC fusion antibody
    • COUP TF interacting protein 2 antibody
    • COUP-TF interacting protein 2 antibody
    • COUP-TF-interacting protein 2 antibody
    • Ctip 2 antibody
    • CTIP-2 antibody
    • CTIP2 antibody
    • hRIT1 alpha antibody
    • hRit1 antibody
    • Radiation induced tumor suppressor gene 1 antibody
    • Radiation induced tumor suppressor gene 1 protein antibody
    • Radiation-induced tumor suppressor gene 1 protein antibody
    • Rit 1 antibody
    • Rit1 antibody
    • zinc finger protein hRit1 alpha antibody
    • ZNF856B antibody
    see all

Anti-Ctip2 antibody [25B6] - ChIP Grade 图像

  • ab18465 staining Ctip2 in mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rat IgG (1/1000) was used as secondary antibody. Staining was improved with citrate antigen retrieval.

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  • Neonatal Mouse Hippocampal Neurons (Harvested at P1, grown 5d in culture on glial cell feeder layer).

    Red is beta tubulin staining.
    Green is ab18465.

  • Neonatal mouse hippocampal neurons stained with ab18465 (top panel - green) and Ctip1 antibody (middle - red). Bottom panel is overlay of ab18465 and Ctip1 antibody staining - yellow indicates co-localisation, green is ab18465 alone and red is Ctip1 antibody alone.


  • Predicted band size : 95 kDa

    Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody.

    Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).

    Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody. Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).
  • ab18465 staining Ctip2 in Guinea Pig Brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.

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  • Immunohistochemistry using ab18465 on Adult Mouse Hippocampus.
  • All lanes : Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465) at 1/500 dilution

    Lane 1 : Mouse brain tissue lysate at 1.5 µg
    Lane 2 : Mouse brain tissue lysate at 1.5 µg
    Lane 3 : Mouse brain tissue lysate at 3 µg

    Secondary
    IRDYE 680-conjugated Donkey Anti-Rat polyclonal. at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 95 kDa
    Observed band size : 100,110 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

    This image is courtesy of an Anonymous Abreview.

    See Abreview

  • Overlay histogram showing Jurkat cells stained with ab18465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18465, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)参考文献

This product has been referenced in:
  • Jara JH  et al. Healthy and diseased corticospinal motor neurons are selectively transduced upon direct AAV2-2 injection into the motor cortex. Gene Ther N/A:N/A (2016). ICC/IF ; Mouse . Read more (PubMed: 26704722) »
  • Leone DP  et al. Compensatory Actions of Ldb Adaptor Proteins During Corticospinal Motor Neuron Differentiation. Cereb Cortex N/A:N/A (2016). Read more (PubMed: 26830346) »

See all 120 Publications for this product

Product Wall

Thanks for passing along the protocol details. They all seem quite reasonable.

If I could I'd just like to ask a few additional questions:

1. Are you still using same vial of antibody?

2. Have you stored the antibody in al...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Embryonic spinal cord)
Antigen retrieval step None
Permeabilization Yes - 0.3%Triton/PBS
Specification Embryonic spinal cord
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Paraformaldehyde
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提交于 Jul 16 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (Kidney)
Permeabilization Yes - 0.1% triton x-100
Specification Kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 Jun 10 2016

Application IHC - Wholemount
Sample Mouse Tissue (Brain, striatum)
Specification Brain, striatum
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提交于 May 23 2016

Application Western blot
Sample Human Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 May 04 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 May 04 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.3% Triton x 100: 30 min
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 May 04 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (cortical neurons)
Permeabilization Yes - 0.25% Triton X-100 in PBS
Specification cortical neurons
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde
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提交于 Jan 07 2016

Application Flow Cytometry
Sample Human Cell (Differentiated hNSCs)
Permeabilization Yes - 0.25% Triton X-100 in DPBS
Gating Strategy Undifferentiated Stem Cells (white)
Specification Differentiated hNSCs
Preparation Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation Paraformaldehyde
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提交于 Aug 18 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Sample Human Cell (human iPS cell derived neurons)
Specification human iPS cell derived neurons
Permeabilization Yes - see blocking buffer
Fixative Paraformaldehyde
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提交于 Jul 10 2014

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