概述

  • 产品名称Anti-Ctip1抗体[15E3AC11]
    参阅全部 Ctip1 一抗
  • 描述
    小鼠单克隆抗体[15E3AC11] to Ctip1
  • 经测试应用适用于: ICC/IF, Flow Cyt, IHC-P, WB, IP, ICC, ChIPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A cocktail of BCL11A fusion proteins.

  • 表位Epitope is in amino terminus of CTIP1/Bcl11a (aa's 1-171).

性能

应用

Our Abpromise guarantee covers the use of ab18688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/50. ab18392-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 91 kDa.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 23576758

靶标

  • 功能Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1.
  • 组织特异性Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development.
  • 疾病相关Note=Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification.
  • 序列相似性Contains 6 C2H2-type zinc fingers.
  • 翻译后修饰Sumoylated by SUMO1.
  • 细胞定位Cytoplasm. Nucleus. Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2810047E18Rik antibody
    • B cell CLL/lymphoma 11A (zinc finger protein) antibody
    • B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody
    • B-cell CLL/lymphoma 11A antibody
    • B-cell lymphoma/leukemia 11A antibody
    • BC11A_HUMAN antibody
    • BCL-11A antibody
    • BCL11A antibody
    • BCL11A B cell CLL/lymphoma 11A (zinc finger protein) isoform 1 antibody
    • BCL11A L antibody
    • BCL11A S antibody
    • BCL11A XL antibody
    • BCL11a-M antibody
    • BCL11AL antibody
    • BCL11AS antibody
    • BCL11AXL antibody
    • C2H2 type zinc finger protein antibody
    • COUP TF interacting protein 1 antibody
    • COUP-TF-interacting protein 1 antibody
    • CTIP1 antibody
    • CTIP1, mouse, homolog of antibody
    • D930021L15Rik antibody
    • Ecotropic viral integration site 9 antibody
    • Ecotropic viral integration site 9 homolog antibody
    • Ecotropic viral integration site 9 protein antibody
    • Ecotropic viral integration site 9 protein homolog antibody
    • EVI-9 antibody
    • Evi9 antibody
    • Evi9, mouse, homolog of antibody
    • FLJ10173 antibody
    • FLJ34997 antibody
    • HBFQTL5 antibody
    • KIAA1809 antibody
    • mKIAA1809 antibody
    • OTTHUMP00000159788 antibody
    • OTTHUMP00000159789 antibody
    • OTTHUMP00000201250 antibody
    • OTTHUMP00000202084 antibody
    • Zinc finger protein 856 antibody
    • ZNF856 antibody
    see all

Anti-Ctip1 antibody [15E3AC11] 图像

  • Overlay histogram showing RAMOS cells stained with ab18688(red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18688, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG3 [MG3-35](ab18394, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Ctip1 antibody [15E3AC11] (ab18688)参考文献

This product has been referenced in:
  • Xu J  et al. Corepressor-dependent silencing of fetal hemoglobin expression by BCL11A. Proc Natl Acad Sci U S A 110:6518-23 (2013). ChIP ; Human . Read more (PubMed: 23576758) »
  • Chan KS  et al. Generation of a genomic reporter assay system for analysis of ?- and ß-globin gene regulation. FASEB J 26:1736-44 (2012). WB . Read more (PubMed: 22267339) »

See all 5 Publications for this product

Product Wall

Unfortunately, ab18688 has not been epitope mapped internally nor externally to our knowledge. Thus, we do not know specifically where the antibody binds within the Ctip1 protein and what isoforms it is capable of recognizing. According to the uniprot ...

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Dear Marti, I am sorry that my previous answer was not sufficient. Thank you contacting us again. We used that antibody between 1:50 and 1:250 for adult mouse hippocampus in frozen and paraffin-fixed sections. The IHC protocol that we recommend can...

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I am sorry to hear that the lab is having trouble with this antibody. A few suggestions I can make are to try incubating the primary antibody over night at 4C. Also, Abcam recommends the ABC Immunohistochemical staining system, so perhaps the lab could...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"