概述

  • 产品名称Anti-CtIP抗体
    参阅全部 CtIP 一抗
  • 描述
    兔多克隆抗体to CtIP
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human CtIP (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: Q99708

  • 阳性对照
    • Human Breast, Colon Myenteric Plexus and Pancreas tissues.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab117722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 101 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations.
  • 疾病相关Seckel syndrome 2
    Jawad syndrome
    Genetic variability in RBBP8 is noted as a factor in BRCA1-associated breast cancer risk (PubMed:21799032). Exhibits sensitivity to tamoxifen in certain breast cancer cell lines (PubMed:18171986).
  • 序列相似性Belongs to the COM1/SAE2/CtIP family.
  • 结构域The PXDLS motif binds to a cleft in CtBP proteins.
    The damage-recruitment motif is required for DNA binding and translocation to sites of DNA damage.
  • 翻译后修饰Acetylated. Deacetylation by SIRT6 upon DNA damage promotes DNA end resection.
    Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control.
    Ubiquitinated (PubMed:14654780, PubMed:16818604). Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteosomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control (PubMed:16818604). Ubiquitinated by RNF138 at its N-terminus (PubMed:26502057).
  • 细胞定位Nucleus. Chromosome. Associates with sites of DNA damage in S/G2 phase (PubMed:10764811). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604).
  • Information by UniProt
  • 数据库链接
  • 别名
    • COM1 antibody
    • COM1_HUMAN antibody
    • CtBP interacting protein antibody
    • CtBP-interacting protein antibody
    • CtIP antibody
    • DNA endonuclease RBBP8 antibody
    • JWDS antibody
    • RBBP-8 antibody
    • RBBP8 antibody
    • Retinoblastoma-binding protein 8 antibody
    • Retinoblastoma-interacting protein and myosin-like antibody
    • Rim antibody
    • SAE2 antibody
    • SCKL2 antibody
    • Sporulation in the absence of SPO11 protein 2 homolog antibody
    see all

Anti-CtIP antibody 图像

  • Lane 1 : Anti-CtIP antibody (ab117722) at 1 µg/ml
    Lane 2 : Anti-CtIP antibody (ab117722) at 2 µg/ml

    Lane 1 : Mouse spleen tissue lystae
    Lane 2 : Mouse spleen tissue lysate


    Predicted band size : 101 kDa
  • Immunohistochemistry Image of Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) stained with anti-CTIP antibody (ab117722).
  • Immunohistochemistry Image of Human Colon, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE) stained with anti-CTIP antibody (ab117722).
  • Immunohistochemistry Image of Human Pancreas, Duct: Formalin-Fixed, Paraffin-Embedded (FFPE) stained with anti-CTIP antibody (ab117722).

Anti-CtIP antibody (ab117722)参考文献

ab117722 has not yet been referenced specifically in any publications.

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