概述

  • 产品名称
  • 描述
    兔多克隆抗体to CTGF
  • 特异性
    This antibody detects recombinant human CTGF by Western Blotting. The band can be blocked by the immunising peptide (ab13741). We have been unable to detect endogenous protein in WB with ab5097.
  • 经测试应用
    适用于: IHC-P, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to Human CTGF aa 150-250 conjugated to keyhole limpet haemocyanin.
    Database link: P29279
    (Peptide available as ab13741)

  • 阳性对照
    • This antibody gave a positive signal in recombinant human CTGF in WB; This antibody gave a positive signal in the following Formaldehyde fixed cell lines:HepG2.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 17028989
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr 1/200.

靶标

  • 功能
    Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
  • 组织特异性
    Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
  • 序列相似性
    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix. Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCN 2 antibody
    • CCN family member 2 antibody
    • CCN2 antibody
    • Connective tissue growth factor antibody
    • Ctgf antibody
    • CTGF_HUMAN antibody
    • Hcs 24 antibody
    • Hcs24 antibody
    • Hypertrophic chondrocyte specific protein 24 antibody
    • Hypertrophic chondrocyte-specific gene product 24 antibody
    • Hypertrophic chondrocyte-specific protein 24 antibody
    • IBP-8 antibody
    • IGF-binding protein 8 antibody
    • IGFBP-8 antibody
    • IGFBP8 antibody
    • Insulin like Growth Factor Binding Protein 8 antibody
    • Insulin-like growth factor-binding protein 8 antibody
    • MGC102839 antibody
    • NOV 2 antibody
    • NOV2 antibody
    see all

图片

  • ab5097 staining CTGF in human bronchus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and an antigen retrieval step was performed using EDTA pH9.0. Samples were then incubated with ab5097 at a 1/400 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/rabbit polyclonal.

    See Abreview

  • ICC/IF image of ab5097 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab5097 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab5097 staining CTGF in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in buffer and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/50 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

    See Abreview

  • ab5097 staining CTGF in Human Hela, cervix cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% normal goat serum for 30 minutes. Samples were incubated with primary antibody (1/750) for 4 hours at 20°C. A Cy3®-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/800).

    See Abreview

  • ab5097 staining CTGF in Pig skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 2 hours at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in blocking buffer) for 24 hours at 4°C. A BHRP-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab5097 staining CTGF in Pig skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS Tween 0.01% + donkey serum 1%) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rat IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview



  • Performed under reducing conditions.

    Exposure time : 10 seconds

    Western blot, on recombinant Human CTGF produced in E. coli, using ab5097 at 1/500.

    Lane 1: 0.01µg Human CTGF
    Lane 2: 0.01µg Human CTGF + CTGF blocking peptide (ab13741)
    Lane 3: 0.1µg Human CTGF
    Lane 4: 0.1µg Human CTGF + CTGF blocking peptide (ab13741)

    Secondary ab: Rabbit polyclonal to Goat IgG HRP ab6741 (1/5000)
    Exposure time: 10 seconds
    Expected molecular weight: 38.3 kDa
    CTGF Blocking peptide (ab13741) used at 1µg/ml.

    Expected molecular weight: 38kDa
    Not yet tested against endogenous CTGF

     

    Secondary antibody - anti-rabbit HRP (ab6721)

文献

This product has been referenced in:
  • Lee CH  et al. Harnessing endogenous stem/progenitor cells for tendon regeneration. J Clin Invest 125:2690-701 (2015). Read more (PubMed: 26053662) »
  • Meng YH  et al. Elevated expression of connective tissue growth factor, osteopontin and increased collagen content in human ascending thoracic aortic aneurysms. Vascular 22:20-7 (2014). IHC ; Human . Read more (PubMed: 23508392) »

See all 11 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Ureter)
Specification
Ureter
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,2 % Trition/PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1%
Username

Ms. Beate Thode

Verified customer

提交于 Feb 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela, Cervix Cancer Cells)
Specification
Hela, Cervix Cancer Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,2 Triton/PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1%
Username

Ms. Beate Thode

Verified customer

提交于 Feb 22 2013

Thank you for your reply and for your cooperation.

As agreed, I have issued a free of charge replacement with one unit of ab6328; the order number is ****.

To check the status of the order please contact our Customer Service team and ...

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En compensation de tous les problèmes rencontrés avec les anti-CTGF, j'aimerais vous offrir un avoir contre la commande originale du ab5097 ou un anticorps de remplacement de votre choix.

Merci de m'indiquer la résolution ...

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J'espère que vous allez bien.

J'aimerais vous informer que mes collègues du laboratoire ont effectué de nouveaux tests en Western Blot avec l'anti-CTGF référence ab5097.

Ils ont essayé diff&ea...

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Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I a...

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Thank you for your message. We appreciate your valuable feedback in the Abreviews.

I can confirm that you will need to place your order using the testing discount code forthe free of charge antibody before 20th August.

I hope this...

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Merci beaucoup pour ce complément d'information.

Je vous contacterai de nouveau dès que j'aurai reçu les résultats du labo pour les anti-CTGF.

Je vous souhaite un très bon week-end.

Thank you very much for your email and the detailed information about your results.

I forwarded your data to our characterisation team who, since your last email, have started to investigate the problem. It has been decided to retest the produ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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