概述

  • 产品名称Anti-CTGF抗体[17-73-F1]
    参阅全部 CTGF 一抗
  • 描述
    小鼠单克隆抗体[17-73-F1] to CTGF
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Cow, Pig, Baboon
  • 免疫原

    Synthetic peptide corresponding to Human CTGF aa 150-250. corresponding to the hinge region of Human CTGF
    Database link: P29279

  • 阳性对照
    • This antibody gave a positive signal when tested against recombinant CTGF protein, and in the following lysates: HeLa whole cell; HEK293 whole cell; Bone marrow (human) tissue; ovary (human) tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号17-73-F1
  • 同种型IgG2
  • 轻链类型lambda
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab94939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 10 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ELISA Use at an assay dependent dilution.

靶标

  • 功能Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
  • 组织特异性Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
  • 序列相似性Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • 细胞定位Secreted > extracellular space > extracellular matrix. Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCN 2 antibody
    • CCN family member 2 antibody
    • CCN2 antibody
    • Connective tissue growth factor antibody
    • Ctgf antibody
    • CTGF_HUMAN antibody
    • Hcs 24 antibody
    • Hcs24 antibody
    • Hypertrophic chondrocyte specific protein 24 antibody
    • Hypertrophic chondrocyte-specific gene product 24 antibody
    • Hypertrophic chondrocyte-specific protein 24 antibody
    • IBP-8 antibody
    • IGF-binding protein 8 antibody
    • IGFBP-8 antibody
    • IGFBP8 antibody
    • Insulin like Growth Factor Binding Protein 8 antibody
    • Insulin-like growth factor-binding protein 8 antibody
    • MGC102839 antibody
    • NOV 2 antibody
    • NOV2 antibody
    see all

Anti-CTGF antibody [17-73-F1] 图像

  • Anti-CTGF antibody [17-73-F1] (ab94939) at 10 µg/ml + CTGF Recombinant Protein (Human) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa


    Exposure time : 4 minutes
  • All lanes : Anti-CTGF antibody [17-73-F1] (ab94939) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 4 : Human ovary tissue lysate - total protein (ab30222)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 44 kDa (possible glycosylated form),50 kDa,60 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

Anti-CTGF antibody [17-73-F1] (ab94939)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall


Le laboratoire vient de me confirmer que l'anticorps monoclonal de souris anti-GTCF, clone [17-73-F1], référence ab94939 est purifié par protéine G. La concentration est de 1 mg/ml.
La fiche technique a ét&ea...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"