概述

  • 产品名称Anti-CTBP2抗体
    参阅全部 CTBP2 一抗
  • 描述
    兔多克隆抗体to CTBP2
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Cow, Monkey, Zebrafish
  • 免疫原

    Recombinant full length protein, corresponding to a region within amino acids 737-927 of Human CTBP2 (NP_073713).

  • 阳性对照
    • Molt-4 whole cell lysate (WB) and HeLa cell (IF)

性能

应用

Our Abpromise guarantee covers the use of ab96107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 106 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
    Isoform 2 probably acts as a scaffold for specialized synapses.
  • 组织特异性Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.
  • 序列相似性Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • 翻译后修饰Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.
  • 细胞定位Nucleus. Cell junction > synapse.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C terminal binding protein 2 antibody
    • C-terminal-binding protein 2 antibody
    • CtBP2 antibody
    • CTBP2_HUMAN antibody
    • ribeye antibody
    see all

Anti-CTBP2 antibody 图像

  • Anti-CTBP2 antibody (ab96107) at 1/500 dilution + Molt-4 whole cell lysate at 30 µg

    Predicted band size : 106 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96107 at 1/100 dilution.
  • Anti-CTBP2 antibody (ab96107) at 1/200 dilution + zebrafish embryo tissue lysate at 40 µg

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/4000 dilution

    Predicted band size : 106 kDa
    Observed band size : 55-60,85-90 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Evgenia Salta

    See Abreview

Anti-CTBP2 antibody (ab96107)参考文献

ab96107 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 50 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing (4°12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Evgenia Salta

Verified customer

提交于 Jul 17 2012

Application Western blot
Sample Zebrafish Tissue lysate - whole (Whole-mount, Larvae, 3 dpf)
Loading amount 40 µg
Specification Whole-mount, Larvae, 3 dpf
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Evgenia Salta

Verified customer

提交于 Jul 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"