概述

  • 产品名称
  • 描述
    兔多克隆抗体to CTBP2
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Cow, Monkey, Zebrafish
  • 免疫原

    Recombinant full length protein, corresponding to a region within amino acids 737-927 of Human CTBP2 (NP_073713).

  • 阳性对照
    • Molt-4 whole cell lysate (WB) and HeLa cell (IF)

性能

应用

Our Abpromise guarantee covers the use of ab96107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 106 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能
    Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
    Isoform 2 probably acts as a scaffold for specialized synapses.
  • 组织特异性
    Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.
  • 序列相似性
    Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • 翻译后修饰
    Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.
  • 细胞定位
    Nucleus. Cell junction > synapse.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C terminal binding protein 2 antibody
    • C-terminal-binding protein 2 antibody
    • CtBP2 antibody
    • CTBP2_HUMAN antibody
    • ribeye antibody
    see all

图片

  • Anti-CTBP2 antibody (ab96107) at 1/500 dilution + Molt-4 whole cell lysate at 30 µg

    Predicted band size : 106 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96107 at 1/100 dilution.
  • Anti-CTBP2 antibody (ab96107) at 1/200 dilution + zebrafish embryo tissue lysate at 40 µg

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/4000 dilution

    Predicted band size : 106 kDa
    Observed band size : 55-60,85-90 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Evgenia Salta

    See Abreview

文献

ab96107 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
50 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing (4°12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Evgenia Salta

Verified customer

提交于 Jul 17 2012

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Whole-mount, Larvae, 3 dpf)
Loading amount
40 µg
Specification
Whole-mount, Larvae, 3 dpf
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Evgenia Salta

Verified customer

提交于 Jul 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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