Anti-CtBP1 (phospho S422)抗体(ab92577)

概述

  • 产品名称Anti-CtBP1 (phospho S422)抗体
    参阅全部 CtBP1 一抗
  • 描述
    兔多克隆抗体to CtBP1 (phospho S422)
  • 特异性Detects endogenous levels of CtBP1 only when phosphorylated at Serine 422.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human CtBP1 around the phosphorylation site of Serine 422 (A-P-SP-P-G).

  • 阳性对照
    • WB: lysate from Jurkat cells pre-treated with TNF (20ng/ml, 30mins); IHC-P: human brain tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab92577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明ELISA: 1/5000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 49 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.
    • 序列相似性Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
    • 翻译后修饰The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.
      ADP-ribosylated; when cells are exposed to brefeldin A.
      Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BARS antibody
      • brefeldin A- ribosylated substrate antibody
      • C terminal binding protein 1 antibody
      • C-terminal-binding protein 1 antibody
      • CTBP antibody
      • CtBP1 antibody
      • CTBP1_HUMAN antibody
      • MGC104684 antibody
      see all

    Anti-CtBP1 (phospho S422) antibody 图像

    • All lanes : Anti-CtBP1 (phospho S422) antibody (ab92577) at 1/500 dilution

      Lane 1 : lysate from Jurkat cells treated with TNF (20ng/ml, 30mins)
      Lane 2 : lysate from Jurkat cells treated with TNF (20ng/ml, 30mins)
      Lane 3 : lysate from Jurkat cells treated with TNF (20ng/ml, 30mins) with immunizing phosphopeptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 49 kDa
    • Left panel: ab92577, at a 1/50 dilution, staining phosphorylated CtBP1 in formalin/ fixed, paraffin embedded Human tissue brain, using Immunohistochemistry.
      Right panel: same, after pre-incubation with the immunizing phosphopeptide.

    Anti-CtBP1 (phospho S422) antibody (ab92577)参考文献

    ab92577 has not yet been referenced specifically in any publications.

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