概述

  • 产品名称Anti-CtBP1抗体
    参阅全部 CtBP1 一抗
  • 描述
    兔多克隆抗体to CtBP1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    3 synthetic peptides (Human) conjugated to KLH

  • 阳性对照
    • Recombinant protein produced in an induced culture of E.coli.

性能

应用

Our Abpromise guarantee covers the use of ab85992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 49 kDa.

靶标

  • 功能Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.
  • 序列相似性Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • 翻译后修饰The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.
    ADP-ribosylated; when cells are exposed to brefeldin A.
    Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BARS antibody
    • brefeldin A- ribosylated substrate antibody
    • C terminal binding protein 1 antibody
    • C-terminal-binding protein 1 antibody
    • CTBP antibody
    • CtBP1 antibody
    • CTBP1_HUMAN antibody
    • MGC104684 antibody
    see all

Anti-CtBP1 antibody 图像

  • All lanes : Anti-CtBP1 antibody (ab85992) at 1/1000 dilution

    Lane 1 : Uninduced (negative control) culture of E.coli
    Lane 2 : Induced culture of E.coli

    Secondary
    anti-Rabbit IgG HRP conjugated at 1/1000 dilution

    Predicted band size : 49 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-CtBP1 antibody (ab85992) at 1/30000 dilution

    Lane 1 : Mouse brain whole cell lysate
    Lane 2 : Mouse brain whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 49 kDa


    Exposure time : 15 seconds

    This image is courtesy of Anonymous Abreview.

    See Abreview

Anti-CtBP1 antibody (ab85992)参考文献

ab85992 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse brain)
Loading amount 10 µg
Specification mouse brain
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 May 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"