The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/62500.
Use a concentration of 1 µg/ml. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
组织特异性Isoform 5A is expressed only in testis (at the protein level). Other isoforms are expressed in several other normal tissues, including brain, muscle and cranial skin. Isoform 5A (at protein level) and isoform 5B are expressed in cutaneous T-cell lymphoma (CTCL) cell lines, colorectal carcinomas, breast carcinomas and melanoma. Isoform 5B, but not isoform 5A, is expressed in head and neck squamous cell carcinoma.
疾病相关Note=Autoantibodies against CTAGE5 are present in several cancer types, including benign meningioma and cutaneous T-cell lymphoma (CTCL).