概述

  • 产品名称Anti-CSTF2T抗体
    参阅全部 CSTF2T 一抗
  • 描述
    兔多克隆抗体to CSTF2T
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Gorilla, Soybean, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 566 and 616 of Human CSTF2T (NP_056050.1).

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

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应用

Our Abpromise guarantee covers the use of ab80275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能May play a significant role in AAUAAA-independent mRNA polyadenylation in germ cells. Directly involved in the binding to pre-mRNAs.
  • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CF 1 64 kDa subunit tau antibody
    • CF-1 64 kDa subunit tau variant antibody
    • CF1 64 kDa subunit, tau variant antibody
    • Cleavage stimulation factor 3' pre RNA, subunit 2 64kDa tau variant antibody
    • Cleavage stimulation factor 64 kDa subunit tau antibody
    • Cleavage stimulation factor 64 kDa subunit tau variant antibody
    • Cleavage stimulation factor 64 kDa subunit, tau variant antibody
    • Cleavage stimulation factor subunit 2 tau antibody
    • Cleavage stimulation factor subunit 2 tau variant antibody
    • CSTF 64 kDa subunit tau antibody
    • CSTF 64 kDa subunit tau variant antibody
    • CSTF 64 kDa subunit, tau variant antibody
    • CstF 64T antibody
    • CSTF2T antibody
    • CSTF2T f antibody
    • CSTFT_HUMAN antibody
    • KIAA0689 antibody
    • TauCstF-64 antibody
    • TauCstF64 antibody
    see all

Anti-CSTF2T antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CSTF2T with ab80275 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-CSTF2T antibody (ab80275) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg


    Predicted band size : 64 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Detection of CSTF2T in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80275 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

Anti-CSTF2T antibody (ab80275)参考文献

ab80275 has not yet been referenced specifically in any publications.

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