Anti-CSRP2/Dlp1抗体(ab65946)
Key features and details
- Goat polyclonal to CSRP2/Dlp1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-CSRP2/Dlp1抗体
参阅全部 CSRP2/Dlp1 一抗 -
描述
山羊多克隆抗体to CSRP2/Dlp1 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Horse, Cow, Opossum -
免疫原
Synthetic peptide corresponding to Human CSRP2/Dlp1 aa 86-97 (internal sequence).
Sequence:C-ERLGIKPESVQP
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阳性对照
- Recombinant Human CSRP2/Dlp1 protein (ab126663) can be used as a positive control in WB. Human heart and kidney lysates. Mouse and rat kidney lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab65946 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65946于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).
A 1 hour primary incubation is recommended for this product. Approx 26kDa band observed in Human Heart and Kidney lysates and in Mouse and Rat Kidney lysates (calculated MW of 21.0kDa according to NP_001312.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem. 2000 Feb 25;275(8):5504-11; PMID: 10681529). |
IHC-P |
Use a concentration of 3 - 6 µg/ml.
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说明 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa). A 1 hour primary incubation is recommended for this product. Approx 26kDa band observed in Human Heart and Kidney lysates and in Mouse and Rat Kidney lysates (calculated MW of 21.0kDa according to NP_001312.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem. 2000 Feb 25;275(8):5504-11; PMID: 10681529). |
IHC-P
Use a concentration of 3 - 6 µg/ml. |
靶标
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功能
Drastically down-regulated in response to PDGF-BB or cell injury, that promote smooth muscle cell proliferation and dedifferentiation. Seems to play a role in the development of the embryonic vascular system. -
组织特异性
Highly expressed in the aorta, but not in heart and skeletal muscle. -
序列相似性
Contains 2 LIM zinc-binding domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 539381 Cow
- Entrez Gene: 1466 Human
- Entrez Gene: 13008 Mouse
- Entrez Gene: 29317 Rat
- Omim: 601871 Human
- SwissProt: Q32LE9 Cow
- SwissProt: Q16527 Human
- SwissProt: P97314 Mouse
see all -
别名
- CRP2 antibody
- CSRP2 antibody
- CSRP2_HUMAN antibody
see all
图片
数据表及文件
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Datasheet download
文献 (1)
ab65946 被引用在 1 文献中.
- Wang J et al. Exosomal miR-27a Derived from Gastric Cancer Cells Regulates the Transformation of Fibroblasts into Cancer-Associated Fibroblasts. Cell Physiol Biochem 49:869-883 (2018). PubMed: 30184548