使用敲除细胞株进行验证RabMAb

Anti-CSN7b抗体[EPR6465] (ab124718)

概述

  • 产品名称Anti-CSN7b抗体[EPR6465]
    参阅全部 CSN7b 一抗
  • 描述
    兔单克隆抗体[EPR6465] to CSN7b
  • 经测试应用适用于: WB, IP, ICC/IFmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human CSN7b.

  • 阳性对照
    • Jurkat, HeLa, HL-60, and HT-29 cell lysates; HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 30 kDa.
IP 1/100 - 1/500.
ICC/IF 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt or IHC-P.
  • 靶标

    • 功能Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
    • 序列相似性Belongs to the CSN7/EIF3M family. CSN7 subfamily.
      Contains 1 PCI domain.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • COP9 Complex Homolog Subunit 7b antibody
      • COP9 Constitutive Photomorphogenic Homolog Subunit 7b antibody
      • COP9 Signalasome Subunit 7b antibody
      • COP9 signalosome complex subunit 7b antibody
      • COPS7B antibody
      • CSN7B_HUMAN antibody
      • JAB1 Containing Signalosome Subunit 7b antibody
      • JAB1-containing signalosome subunit 7b antibody
      • SGN7b antibody
      • Signalosome subunit 7b antibody
      see all

    Anti-CSN7b antibody [EPR6465] 图像



    • Predicted band size : 30 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: CSN7b knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab124718 observed at 32 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab124718 was shown to specifically react with CSN7b when CSN7b knockout samples were used. Wild-type and CSN7b knockout samples were subjected to SDS-PAGE. ab124718 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-CSN7b antibody [EPR6465] (ab124718) at 1/10000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : HL60 cell lysates
      Lane 4 : HT-29 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 30 kDa
    • ab124718, at 1/50, staining CSN7b in HeLa cells by Immunofluorescence.

    Anti-CSN7b antibody [EPR6465] (ab124718)参考文献

    ab124718 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (HAP1 cells)
    Gel Running Conditions Non-reduced Denaturing (4-12% Bis-Tris gel)
    Loading amount 50 µg
    Specification HAP1 cells
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    提交于 Oct 10 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"