Anti-CSN1 (phospho S474)抗体(ab111894)

概述

  • 产品名称Anti-CSN1 (phospho S474)抗体
    参阅全部 CSN1 一抗
  • 描述
    兔多克隆抗体to CSN1 (phospho S474)
  • 特异性ab111894 detects endogenous levels of CSN1 only when phosphorylated at serine 454.
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human CSN1 around the phosphorylation site of serine 474 (E-G-SP-Q-G).

  • 阳性对照
    • Human Brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab111894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Suppresses G-protein-and mitogen-activated protein kinase-mediated signal transduction.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the CSN1 family.
    Contains 1 PCI domain.
  • 结构域The PCI domain is necessary and sufficient for the interactions with other CSN subunits of the complex. Mediates the interaction with CAPN8.
    The N-terminal part (1-216), which is not required for deneddylating activity and CSN complex formation, is nevertheless essential for other aspects of CSN complex function, such as repression of c-fos/FOS expression.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COP9 signalosome complex subunit 1 antibody
    • COP9 signalosome subunit 1 antibody
    • COPS1 antibody
    • CSN 1 antibody
    • CSN1_HUMAN antibody
    • FUS6/COP11, Arabidopsis, homolog of antibody
    • G protein pathway suppressor 1 antibody
    • GPS-1 antibody
    • Gps1 antibody
    • JAB1-containing signalosome subunit 1 antibody
    • Protein GPS1 antibody
    • Protein MFH antibody
    • SGN1 antibody
    • Signalosome subunit 1 antibody
    see all

Anti-CSN1 (phospho S474) antibody 图像

  • ab111894 at 1/50 dilution staining CSN1 in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

Anti-CSN1 (phospho S474) antibody (ab111894)参考文献

ab111894 has not yet been referenced specifically in any publications.

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