概述

  • 产品名称Anti-CSN1抗体
    参阅全部 CSN1 一抗
  • 描述
    兔多克隆抗体to CSN1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human COP9 signalosome complex subunit 1 encoded within exons 13 and 14 (LocusLink ID 2873).

  • 阳性对照
    • Whole cell lysate from mock transfected or CSN1 transfected HEK293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab10413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Suppresses G-protein-and mitogen-activated protein kinase-mediated signal transduction.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the CSN1 family.
    Contains 1 PCI domain.
  • 结构域The PCI domain is necessary and sufficient for the interactions with other CSN subunits of the complex. Mediates the interaction with CAPN8.
    The N-terminal part (1-216), which is not required for deneddylating activity and CSN complex formation, is nevertheless essential for other aspects of CSN complex function, such as repression of c-fos/FOS expression.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COP9 signalosome complex subunit 1 antibody
    • COP9 signalosome subunit 1 antibody
    • COPS1 antibody
    • CSN 1 antibody
    • CSN1_HUMAN antibody
    • FUS6/COP11, Arabidopsis, homolog of antibody
    • G protein pathway suppressor 1 antibody
    • GPS-1 antibody
    • Gps1 antibody
    • JAB1-containing signalosome subunit 1 antibody
    • Protein GPS1 antibody
    • Protein MFH antibody
    • SGN1 antibody
    • Signalosome subunit 1 antibody
    see all

Anti-CSN1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CSN1 with ab10413 at 1/1000 (1µg/ml). Detection: DAB.
  • Detection of Human CSN1 by Western Blot. Samples: Whole cell lysate (50 mcg – E; 10 mcg – T) from mock transfected (E) or CSN1 transfected (T) HEK293T cells.

Anti-CSN1 antibody (ab10413)参考文献

This product has been referenced in:
  • Oh W  et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). Read more (PubMed: 16624822) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. This product will not work for E. nidulans. The epitope recognized by ab10413 maps to a region between residue 400 and the C-terminus (residue 471) of human G protein Pathway Suppressor 1 using the numbering given in Sw...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"