存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg +2 and Ca +2), 150mM Sodium chloride, pH 7.4
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纯度 Immunogen affinity purified
纯化说明 Affinity purified from rabbit antiserum by affinity chromatography using
epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 51 kDa.
功能 Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
组织特异性 Expressed in lung and macrophages.
序列相似性 Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.
翻译后修饰 Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
细胞定位 Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
Information by UniProt
C SRC antibody
C SRC kinase antibody
C src Tyrosine Kinase antibody
Anti-CSK (phospho S364) antibody 图像
Western blot - CSK (phospho S364) antibody (ab61782)
All lanes : Anti-CSK (phospho S364) antibody (ab61782) at 1/500 dilution Lane 1 : extracts from HeLa cells, treated
with PMA (125ng/ml, 30mins) with no immunizing peptide Lane 2 : extracts from HeLa cells, treated
with PMA (125ng/ml, 30mins) with immunizing peptide Predicted band size : 51 kDa Observed band size : 55 kDa ( why is the actual band size different from the predicted?)
Anti-CSK (phospho S364) antibody (ab61782)参考文献
This product has been referenced in:
et al. Receptor desensitization and blockade of the suppressive effects of prostaglandin E(2) and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes. Cancer Immunol Immunother 60:111-22 (2011).
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